Preparation And Application Of Capillaries Modified By Polymers With Different Charge

一,Preparation and characteristic of capillary columns modified by polyacrylamide with different groupsAllylamine or sodium vinyl sulfate was added into the solutions of acrylamide and N,N'-methylenebisacrylamide to make mixed monomer solutions. These solutions were respectively introduced in methacrylsiloxane-modifidied capillaries. In situ polymerization was initiated by thermally induced radicals. By altering the addition amounts of allyamine or sodium vinyl sulfate, differently charged polymeric layers were obtained and the restrainable effect of proteins'adsorption was perfect. It is convenient to adjust EOFs in the columns and make it satisfied our actual requirement by this way.二,Application of different polymer modified columns in Capillary Zone Electrophoresis and Micellar Electrokinetic Capillary ChromatographyComparing capillary zone electrophoresis of five different columns modified by polymers with different charge, we find that one basic polymer modified column shows perfect separate performance. Further comparison revealed that the columns modified with a solution containing 0.15mol/L acrylamide, 0.03mol/L N,N'-methylenebisacrylamide and more than 1mol/L allylamine exhibited good resolution. This preparation method of modified column has many strongpoints, such as manipulation convenient, cheap, high success rate and so on. In the pattern of Micellar Electrokinetic Capillary Chromatograghy and optimized the separate conditions, the electroosmotic flow in the capillary was adjusted effectively and the adsorption of proteins was restrained via used the chemically bonded columns with different charge and used sodium dodecylsulphate (SDS) as the micellar phase. Separated egg white protein in this pattern, we got the perfect effect. Further comparison with dynamic coat method—a new method developed in the recent years revealed that this method had better resolution and reproducibility.三,Isoelectric Focusing with an Open Tubular Immobilized pH GradientHere we research a new method to achieve an immobilized pH gradient in the form of open tubular style. Four monomers, allylamine, acrylamide, N,N'-methylenebisacrylamide, and acrylic acid were used to make a series of solutions with different pH values, from 3.37 to 10.41. In the situ polymerization of the different pIs. The polymers not only modified the capillaries but also acted as focusing media. A rapid, CAs-free CIEF is available in this open tubular immobilized pH gradient. At the same time, nonspecific adsorption and protein precipitation are limited because of the hydrophilicity and open tubular feature of the polymers. Comparing with their packed partners, we find the open tubular stationary phases retain analytes by just a thin polymer film and exhibit a significant performance advantage.