| The phenomena of nonequilibrium and nonlinear in the nature has attracted more and more reasearchers to study deeply. The research of chemical oscillation was fruitful both in theoretical and in experimental chemical kinetics in recent years. Oscillating chemical reaction exhibits exotic behavior and unusual mechanisms.Consequently, they have been considered a useful tool for the determination of trace amounts of chemical compounds. In this paper, we mainly studied the design of a new oscillating system and its application on analytical determination. The catalyst in this oscillating reaction is macrocyclic complex [CuL](ClO4)2 , the unsaturated ligand L in the complex [CuL](ClO4)2 is 5,7,7,12,14,14-hexemethyl-1,4,8,11- tetraazacyclotetradeca-4,11-diene. We applied the novel oscillating reaction to the determination of Tyrosine,Tryptophan and Cysteine,and satisfactory results were obtained. The main content consists of five parts:1. Chemical oscillating reaction and its application on ananytical chemistryIn this section, we described the basic thermodynamic theory of oscillating chemical reaction and experimental development of these reaction, and we introduced several usual oscillating chemical reaction and their mechanisms. Some oscillating chemical reaction that were used in analytical determinations, especially B-Z chemical oscillations, are reported in details. The research progress in applications for analysis is discussed systematically through representative examples. Finally, we mentioned the importance of these reaction in the field of chemistry, biochemistry and engineering.2. Systhesis of macrocyclic complex [CuL](ClO4)2 and the determination of TyrosineThe catalyst macrocyclic complex [CuL](ClO4)2 was synthesized according to literature and was identified by I.R. spectrum,elemental analyses. Then, the novel oscillating reaction was applied to the determination of Tyrosine. This analytical method, which equipped with a simple instrument, provides a high selectivity for accurate determination of Tyrosine.3.Determination of Tryptophan on [CuL](ClO4)2 catalyzed oscillating reaction and study of its mechanismIn this investigation, a new analytical method for the determination of Tryptophan based on its perturbation on the B-Z oscillating chemical system was proposed. The method depends on the relationship between the change in the oscillating mutation amplitude of the reaction system and the concentration of Tryptophan in the range of 3.148×10-4 mol/L to 1.478×10-6 mol/L, with a correlation coefficiend of 0.9984. The cyclic voltammetry was applied to study the mechanism of Tryptophan with the oscillating reaction.4. Determination of Cysteine and study of its mechanismA novel and convenient method with good selectivity and hige sensitivity for the determination of Cysteine based on the B-Z oscillating chemical reaction has been presented. The optimum condition for the determination has also been investigated. In the B-Z reaction system,when the sample was injected, the mutation of the amplitude fits a second-order polynomial equation to the logarithm of the concentration of Cysteine in the range 2.454×10-7 mol/L to 3.388×10-3 mol/L very well(r=0.99449)5.The work which is uncompleted and ready for accomplishmentSome ideas of the work which is uncompleted and ready for accomplishment are mentioned briefly in this section. |
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