Proton Tranfer Assisted By External Electric Field For Rhodobacter Sphaeroides Hydrogen-photoproduction

Hydrogen become the most promising alternative energy sources because of its renewable, clean and efficient and other prominent advantages. The Photosynthetic Bacteria can use the energy of light and eliminate the organic matter to product hydrogen, and the speed of hydrogen production quickly, the purity of hydrogen hightly and so on, but present research was still at the laboratory stage, the hydrogen productivity was still low, the hydrogen-photoproduction technological is restricts seriously on development. In order to raise the productivity of Photosynthetic Bacteria, we considered that provide the proton by the outside system to the hydrogen-photoproductin system of the Photosynthesis Bacterium to enhance the hydrogen production. This article use the Double Chamber Reacter to research, the organic matter oxygenolysis release protons in the anode chamber, the protons through the Proton Exchange Membrane(PEM) to the cathode chamber by electric field assisted, Photosynthetic Bacteria use these protons to produce hydrogen in the cathode chamber, thus to enhances hydrogen production.The object of study is Rhodobacter sphaeroides. First, we were studied the optimized condition of R. sphaeroides in growth and hydrogen production, as well as studied the performance of mixed substrate to product hydrogen; then carry on the external electric field to domesticate R. sphaeroides, cause it to adapt to the micro electric field circumstances, and domesticated R. sphaeroides has analyzed the carbon source metabolism influence from external electric field by the Micro Plate of Biolog GN of Biolog GN; finally, we studied about the external electric field assiste proton tranfer for Rhodobacter sphaeroides hydrogen-photoproduction, and the ecological relationship on hydrogen production between R. sphaeroides and others bacteria. The results show that:(1) R. sphaeroides grows well in pH 7.0～8.0, the strength of illumination 30001x, the temperature between 30～35℃, and anaerobic light condition.(2) The optimum condition of R. sphaeroides hydrogen-photoproduction is:48h seed fluid, pH7.0～7.5, light intensity for 3000～70001x, temperature for 30℃～35℃.(3) R. sphaeroides can use many kinds of different carbon source to produce hydrogen, but hydrogen production activity to substrate have the selectivity, glucose has the biggest hydrogen output and the average hydrogen production rate, reached 2.625mL/L and 16.6mL/L/h, sodium succinate has the maximum substrate conversion rate to reach 54.8%; when sodium succinate and glucose according to 15:15(mmol/L) mixed to produce hydrogen, the hydrogen output, the average hydrogen production rate and the substrate conversion rate enhanced 35.3%,35.5% and 35.3%, separately.(4) When use the Double Chamber Reacter to research hydrogen production from R. sphaeroides, the anode chamber with the anode domesticated R. sphaeroides as the degeneration bacteria, the light intensity for 30001x anaerobic culture, sodium succinate as the supplies carbon source, external voltage 0.3V, the result of R. sphaeroides hydrogen production to be best in the cathode chamber, compared with the pure cuture, the hydrogen output to enhance 85.7%. Proved R. sphaeroides can use the protons which the sur-system provided to produce hydrogen.(5) The results of external electric field domesticated R. sphaeroides carbon source metabolism analysis by Micro Plate of Biolog GN is:the anode chamber domesticated R. sphaeroides the Sole carbon source utilization (S) increased 9, the cathode chamber domesticated R. sphaeroides S has the drop slightly, but the Average well color development (AWCD) of them has the distinct enhancement, explained that the external electric field can be to enhance the metabolism activity of R. sphaeroides.(6) R. sphaeroides assisted by the Double Chamber Reacter to produce hydrogen to be able to enhance hydrogen output, the mechanism may summarize that:The anode chamber to provide the external protons for the hydrogen-production system of R. sphaeroides, simultaneously, the protons also can be to adjust the pH environment; in addition, the external electric field can be to enhance the metabolism activity of R. sphaeroides.(7) When anode chamber joins R. sphaeroides, R. palustris, E.coli DH5α, E.coli BL21and H. salinarum lives in fossilization salt to SA-PC, separately; the hydrogen-production ability of R. sphaeroides to be able to enhance in the cathode chamber; R. sphaeroides in the anode chamber is the best; Moreover, the anode chamber has not presented the acidified phenomenon.