| ECL has attracted extensive studies both in fundamental aspects as well as in analytical applications. As a detection method, ECL provides high sensitivity through the generation of an optical signal triggered by an electrochemical reaction. The ruthenium tris(bipyridine) (Ru(bpy)32+) and its derivatives are no doubt the most extensively studied complexes. However, these expensive in the analysis of reagent consumption will continue to bring high-cost analysis. Therefore, such ECL reagent immobilized on a cause for great interest. For example, Ru(bpy)32+ has been immobilized in Langmuir–Blodgget films, self-assembled films, polymer films, sol–gel composites, however, most ruthenium complexes are water soluble, sensitive and the detection limits obtained by these ruthenium derivatives are not satisfactory. Due to metal iridium complexes and bipyridine ruthenium complex is similar to the ground, excited state Eh, and most iridium complexes are insoluble in water, thus, the research and applications of solid-state Electrochemiluminescence of iridium complexes is most important. So this paper investigated the immobilization of this ECL reagent. The main results were given as follows:1. Constructed the multi-walled carbon nanotubes/Ployvinyl alcohol/(pq)2Ir(N-phMA) modified electrode. The method of carbon nanotubes dispersion in the solution was modified, introduce polyvinyl alcohol as the dispersant. The MWNT/PVA/(pq)2Ir(N-phMA) modified electrode was constructed by dropping the modified solution, using TPA as coreactant, optimized for the amount of MWNTs, (pq)2Ir(N-phMA), modified solution in the film, pH and scan rate. Make modified electrode to achieve the best efficiency.2. We report herein that ammonium ion, can be sensitively detected as a coreactant in the ECL reaction of a novel iridium complex (pq)2Ir(N-phMA). Research the mechanism of NH4 + as a reactant to participate in the ECL reaction. modified carbon electrode detect a lots of Amino acid and other co-reactants, generalize the ECL law of aromatic amines, fatty amine, inorganic ammonium. Meat release ammonia in the process of corruption, as time to increase, amount of ammonium was increased. In this paper, we collect air samples to test for ammonium in the air, to judge the degree of freshness of meat.3. (bpy)2Ru(phenCl4)(PF6)2 has been synthesized and the structure of the complex was characterized by element analysis, IR spectroscopy and proton NMR spectroscopy. The complex displays absorptions in the UV and visible regions, with visible maximum absorption at 440 nm, a typical metal-to-ligand charge transfer (MLCT) transitions. Photoluminescence emission also has the characteristics of the MLCT transitions with the maxima wavelengths ranged from 630 to 649 nm depending on the different solvents. The electrochemiluminescence (ECL) of the complex was found to be less dependant on the pH, Furthermore the background ECL signal of the complex did not increase even in a strong alkaline condition. Which was distinctly different from that of ruthenium tris(bipyridine) complex, and is not suitable for used in alkaline condition. |
