| Semicarbazide is the coproduction which thermal decomposition by addition to food azodicarbonamide..As a kind of new flour amendment azodicarbonamide have two function of oxidation and ablation.It is a kind of fast effective on flour.The nominate dosage is 10-20g/1000kg on flour. Azodicarbonamide noxious property is GRAS(FDA,1985),ADI:0～45 mg/kg(FAO/ WHO,1985).As a kind of farina tritici deal agent and fast-fermentation on hake food,the max permission dosage (FAO/WHO,1965)is 45mg/kg.It will generate semicarbazide when the flour with azodicarbonamide was high temperature baked,especially on the surface,may reach to 0.2mg/kg.Azodicarbonamide is also a kind of industry blowing agent,which is used on pabular packing material.Ascend to 2003 the EFSA early warned to international,identification the semicarbazide is semicarbazide on mice,and asserted it was not only to harm the human health.But in many countrys,including china,azodicarbonamide still be used as food addition and packing material.The experiment to study the venenosus affection on mice,in order to affect the toxicity information.meanwhile,it will provid relative theory evidences of it's deploy on food.The study according to nation food safety evaluation on toxicology procedure and method,to carry out the tox research of Semicarbazide of Food Additive By-production.The consequence is:1.The consequence of accumulation toxicity test of semicarbazide is mid accumulation toxicity.2.In the subchronic toxicity test,along with cumulation cumulation accretion,the weight growth slow-moving,especially female'sweight growth retardation or body weight loss,demonstrated the semicarbazide accumulatee to a dosage generated tox effective;The study concluded that semicarbazide has certain effect on coeur,liver and kidney through the study on organ index and blood biochemical index.and checking of pathology and histology.3.The generation toxicity study of semicarbazide on mice,the experiment group compared with negative control group,high dose group and mid dose group's teratospermia rate is patency higher,it means semicarbazide has generation toxicity;AC-PH enzymatic activity is predominance higher on low-dose group mice on didymus,it means low-dose is the sensitiveness dose of capacitation and other active,probably the reason of male sterile.4.CA analytical test of semicarbazide,Microcleus test of born marrow cell in mice,the positive control (♀28.5±0.85‰,(?)29±0.65‰),high dose(♀28.5±1.5‰,(?)30±0.71‰),mid dose group(♀27±1.31‰, (?)20±1.08‰) low-dose group((?)12±0.71‰) compared with negative control(♀2.5±0.25‰,(?)3±0.5‰) significant deviation(P＜0.01) micronuclear rates significant higher than negative control group,low-dose group female mice(♀8±1.08‰) compared with negative control group is significant difference,it means semicarbazide has mutagenic action;Chromosome aberration test of born marrow cell:chromosome and chromatid break is chief,the aberration rate aberration rate group(30.75%) and mid dose group(23.63%) is significant higher than negative control group(1.38%),so,experiment consequence is masculine, semicarbazide is chromosome clastogen.5.Conceptotoxicity study of semicarbazide:Compared with negative control group,there was no marked difference about weight and length of offspring.Three experimental groups all didn't have malformation in the embryo appearance,skeleton,cartilage and viscera.6.Immune tox study of semicarbazide:in this experiment,male mice's thymus and spleen ponderal index (thymus 33.44±3.48mg/10g.bw,spleen 49.26±4.35 mg/10g.bw) is diminish,it means the male mice diminish breakdown,while female mice is no effection. |
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