Research On Extraction And Purification Technology Of Glycyrrhizic Acid And Flavanone In Plant Licorice

The licorice for medicinal purposes part is the root of the leguminosae licorice the (Glycyrrhiza) plant.The licorice confirmed that it has makes up spleen profit gas,the clearing heat and detoxication,to dispel phlegm relieves a cough,the emergency to stop pain,effect is well distributed with other medicine,may use in treating the weakness of spleen and stomach,the tired asthenia,the palpitation to pant,to cough the multi-phlegm,the abdominal cavity and four extremity ache and so on sickness, and alleviates other medicines with it the toxicity.The wild licorice resources is day by day deficient as well as the licorice demand increases suddenly day after day,causes the manual plant licorice to be valued people's day by day.To planted the licorice the quality and the vegetal period proposed the new request,this topic selected the Ningxia Huian licorice planter Limited company to plant the licorice is the object of study,experiment in researching the plant licorice get the glycyrrhizic acid content changing of the different growth age;Glycyrrhizic acid and Glycyrrhiza flavanone microwave extraction process;and the glycyrrhizic acid separation purification craft,obtains the high content glycyrrhizic acid.The experiment establishes the best comprehensive utilization process of the plant licorice.(1)Uses the HPLC law the analysis method,carries on the determination to the licorice plant glycyrrhizic acid content,the definite root glycyrrhizic acid content is highest,but the stem and the leaf middle licorice acid content is low,especially the main root spot licorice acid content achieves 3.08%,we discovered that the planter licorice achieved one year half a lifetime lived to two years has already reached five year wild licorice glycyrrhizic acid content and the glycyrrhizic acid soap glucoside content,and the planter licorice achieved for medicinal purposes standard for picking.(2)Screens the method is the microwave auxiliary extraction,the extraction resolver uses 10%ethyl alcohol the mix solution,the microwave extraction process are:The liquid-solid compared to 1:10,the ammonia water quantity is 1%,the extraction time take the 1.5h extraction time as the most appropriate time,the microwave power is 700W,in the glycyrrhizic acid extraction technological process, the acid sinks the settlement time is to be most reasonable in 8h,extraction number of times according to production arrangement is 3 times to be most reasonable,The glycyrrhizic acid content after the best process conforms to the national standards.(3)The macroporous adsorption resin is used for separation and depuration glycyrrhizic acid,Experimental chooses D-101 to take the polymeric adsorbent,the biggest adsorptive capacity is 171.2(mg the glycyrrhizic acid/g resin),the desorption rate is 85.22%,the chromatographic analysis process's optimized conditions are:On the column fluid density is 9mg/mL,the pH value is 6,the desorption uses 4BV10% ethyl alcohol and 1BV/h speed of flow carries on the elution,the elution product glycyrrhizic acid purity reaches 81.72%,uses the activated charcoal to decolorize, the purity achieves 90%.(4)Uses the licorice dregs resources is raw material,in the licorice dregs total flavanone extraction process,the optimum extraction technological conditions are: 80%ethyl alcohol,liquid solid compared to 10:1,microwave power 300W, microwave auxiliary backflow 1h.Total flavanone receiving rate achieves 0.408% after the experimental verification licorice dregs.