Study On Production Process Of Glucose Tolerance Factor Puerarin Functional Milk And Its Intervention Effect On Impaired Glucose Tolerance Mice

Objective: To study theory advantages in the Chinese medicine "Preventive Treatment of Disease" , using modern biological technology, utilize the biotransformation function of cows transforming the function of milk with high-activity glucose tolerance factor (GTF) form chromium-enriched yeast. Preparating GTF Puerarin function milk form Chinese medicine active ingredients Puerarin, to observe the role of intervention to the function of GTF Puerarin milk on Grape impaired glucose tolerance (IGT) in mice, through skeletal muscle GLUT-4 gene expression to clarify its molecular mechanism of intervention on IGT, to provide new ideas for traditional Chinese and western medicine in the treatment of IGT intervention.Methods: (1) Dividing randomly the cows into four groups, to add the chromium-rich yeast to dairy cattle feed at different dose groups respectively, through the detection of chromium content in milk to conclude the relationship between milk chromium content and chromium-rich yeast additive dosage.(2)Choosing GTF functions milk, ordinary milk, chromium-rich yeast and metformin to lavage stomatch to impaired glucose tolerance (IGT) mice model induced by D-galactose peritoneum injection, to compare the intervention role on the IGT mice between the milk GTF functions and each control group.(3) Extracting Pueraria lobata Puerarin form wilding Puerarin with GTF function of milk in proportion to the preparation of GTF functions Puerarin milk, to achieve initially GTF Puerarin functional milk production process.(4)Adopting D-galactose in mice induced by intraperitoneal injection of IGT model, GTF Puerarin milk will function by gavage administration in different doses, to observe each group of mice IGT fasting blood glucose (FBG),oral glucose tolerance test (OGTT), insulin tolerance test (ITT),serum fasting insulin (FINS) and insulin resistance index (HOMA-IR) levels.(5)Applying Reverse transcriptase-polymerase chain reaction (RT-PCR) technology,though detecting Puerarin by GTF function of different doses of milk after treatment IGT intervention group of mice skeletal muscle glucose transporter 4 (GLUT-4) gene expression level, to explain the possible mechanism of GTF Puerarin functional milk interfere role in IGT mice.Results: (l)There were significant correlations between Chromium content of milk and volume of dairy feed additive chromium-rich yeast.(2) Compared with ordinary milk group, chromium-rich yeast group, GTF functions milk intervention on the role of IGT in mice increased.(3)GTF Puerarin functional milk of mice IGT fasting blood glucose (FBG) had no significant effect, reduced oral glucose tolerance test (OGTT) 2 hours after glucose (2hBG), increased insulin tolerance test (ITT) after the insulin sensitivity, lowered fasting insulin (FINS) and insulin resistance index (HOMA-IR) levels.(4)GTF Puerarin functional milk IGT mice significantly increased skeletal muscle glucose transporter 4 (GLUT-4) gene expression level.Conclusion: (1)Chromium diet dairy organism biology may be translated into highly active chromium enrichment in the milk, the milk in the chromium content and chromium content of feed was positively correlated.(2) Function of milk in the milk may be associated with chromium in a variety of amino acids formed after complexation glucose tolerance factor (GTF), regulation of glucose metabolism on the IGT intervention played a role in mice.(3) After Puerarin and function of milk composed with GTF assembling, the efficacy had a synergistic effect.(4)The role of mechanisms GTF Puerarin functional milk intervention on the treatment of IGT in mice was possible increasing the peripheral target cells the expression of GLUT-4mRNA to enhance the uptake of glucose Organize, improved insulin sensitivity.