Study On Degradation Characteristics Of Penicillium Expansum Link F12 Degrading Metsulfuron-methyl

In the study,one degrading strain F12(Penicillium expansum Link) was utilized and mensurated the rate of metsulfuron-methyl-degrading by HPLC.Its growth characteristic, degrading ability were also investigated.The brief results are follows.Through different method bioassay and HPLC,the experiment showed:HPLC is more suitable for fast,accurate determination metsulfuron-methyl residues..By F12 culture based on utilizing metsulfuron-methyl as sole carbon source in inorganic salt solution,as a result,F12 adapt to degrade metsulfuron-methyl in the original concentration of 30mg/L,the inculation of 8%,the pH of 6.5 and the temperature of 28℃circumstances.In the fittest conditon,the metsulfuron-methyl degrading curve described by first order kinetics reaction equation linear regression,metsulfuron-methyl degradation kinetics follow the first order kinetics:C_t=30.62·e^-0.0202t,Its square of correlation coefficient was 0.9511.87.69%of metsulfuron-methyl was removed after 25d In fittest culture condition.The soluble enzymes from cell-free extracts of strain F12 can degrade 85.26%of metsulfuron-methyl in 72h.This shows that the soluble enzymes were the primary removal enzymes.92.16%and 88.57%of the initially added 30 mg/L metsulfuron-methyl was removed after 25d for natural dried fresh soil and sterilized soil inoculated with strain F12.In contrast,Only 14.81%and 8.05%was removed in uninoculated natural dried fresh soil.Therefore,metsulfuron-methyl removal improved significantly in soils inoculated with strain F12.