| Liquid core waveguide(LCW) tube is made from material that have special optical character. It can greatly advance the sensitvity of common photometry. People have noticed it because of its simpleness and low consumption and its application also develop fleetly. In this work , we improve the sensitvity of traditioal photometry based on the LCW and light intensity difference technique, and come true to determineβ2-microglobin, rHu IFN-α, CEA, and AFP in human serum. The study includes two parts.Chapter One Summarization1. In this part, the development of micro-flow injection and biosensor and application were discussed in detail.2. In the sencond part, the characteristics of liquid core waveguide as well as its applications in analytical chemistry was introduced.Chapter two Research reports which are compsed of three components as follows:1. Detection ofβ2-microglobin in Serum and Urine by Liquid Core Waveguide Array ChipIn this study, a detection system with liquid core waveguide array chip for immunoassay was put forward, which combined the technique of light intensity difference to enhance sensitivity of ELISA. The linear rang for HRP was 1.0×10-11g/mL～9.0×10-13g/mL with a detection limit of 3×10-14 g/mL. The RSD was less than 1% (c=3×10-13g/mL,n=11),The linearity range low limit and detection limit were lower 100 times than usual spectrophotometry. This method was applied to detectβ2-microglobin in serum and urine, and the result of sample analysis matched the standard method well.2. Detection of rHu IFN-αin serum by Liquid Core Waveguide Array ChipA sensitive, simple and rapid detection system with liquid core waveguide array chip for immunoassay has been developed, which combined the technique of light intensity difference to enhance the sensitivity of the enzyme-linked immunosorbent assay (ELISA) for approximately 100 times. Under the optimum conditions, the increased light intensity difference was proportional with the concentration of HRP in the range of 3.0×10-8～3.0×10-7 g·L-1, and the detection limit was 5×10-9 g·L-1 (3r). This system has been successfully applied to detect recombinant human interferon-α(rHu IFN-α) in serum. The results obtained compared well with those by ELISA.3. Detection of AFP in human serum by Liquid Core Waveguide Array ChipAFP was determinde by LCW and light intensity difference technique with the method of ELISA, and ultra bright blue LED worked as spectroscopic soure. In the optimal experiment condition, we carried out control experiment about traditional spectrophotometer and LCW, and the result of sample analysis matched the standard method well.4. Detection of CEA in human Serum by Liquid Core Waveguide Array ChipA detection system with liquid core waveguide array chip for immunoassay was put forward, which combined the technique of light intensity difference to enhance sensitivity of ELISA. The HRP of linearity range low limit and detection limit were lower 100 times than usual spectrophotometry. This method was applied to detect CEA in serum, and the result satisfied.
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