Research On The Anti-bacterial Finish Of Wool Fabric By Immobilized Lysozyme

Lysozyme was a specialized enzyme for disruption of microbial cells. It was widely existed in the animals, plants and microorganisms with so many advantages, such as perfect antibacterial performance, safe, non-toxic, thermal stability, and extensive utilizing range. It had been widely applied in the food, pharmaceutical, biotechnology, cosmetics and other industries. Many scholars both at home and broad did large numbers of studies on immobilization of lysozyme. They immobilized lysozyme on different types of supports and had different purposes. But there were a few reports on immobilization of lysozyme on fabric for establishing a new method of antibacterial textiles.The processes of lysozyme immobilized on wool fabric were studied in the paper and the optimal conditions of immobilization were obtained. On this basis the characterizations of immobilized lysozyme that was immobilized under optimal condition were researched. The main contents included the optimum temperature and pH, thermal stability, optional stability, storage stability and the effects of some additives on activities of immobilized and free lysozyme. Finally, the article gave a research on antibacterial effect of the wool fabric by immobilized lysozyme.The results showed that the optimal conditions for immobilization of lyzozyme with wool fabric and glutaraldehyde were as follows: Wool fabric was cross-linked with 0.2% glutaraldehyde for 2 hours, lysozyme 5g/L, immobilization time 6h, immobilization pH 7.0. The optimal conditions for immobilization of lyzozyme with wool fabric by adsorption were as follows: lysozyme 5g/L, immobilization pH 7.0, adsorption time 0.5h.The optimum temperature of immobilized lysozyme was the same as the free enzyme. The optimum pH of immobilized lysozyme was 7.0, comparing with the free enzyme presented the optimum pH at 6.0. Under a certain condition, the immobilized lysozyme was reusable. The thermal and storage stabilities and the effect of metal ions on activity of immobilized lysozyme were better than those of free enzyme. Scanning electron microscopy, infrared-ray test techniques and the Bradford method were used to characterize lysozyme immobilization. At last, the antibacterial effect was assessed by using shake flask test. The reduction percentage of Staphyllococcus aureus by cross-linking got to 80.95%, and the reduction percentage by adsorption was 56.19%.The washing resistance by cross-linking was well.