Extraction Of The Antiseptic Of Cosmetic From Honeysuckle's Leaves

In this paper, extract of Honeysuckle's Leaves was tested for its antimicrobial activities, including investigation of its antimicrobial spectrum, MIC value. The processing conditions of leaching active substances from Honeysuckle's Leaves were optimized with the orthogonal design test. The sensitivity to universal factors of the extract was studied. At the same time, the isolation and purification of the antimicrobial components of Honeysuckle's Leaves were also studied .The water and ethanol extracts of Camphotree Leaves, safflower, Honeysuckle and its leaves were obtained by refluxing with both water and ethanol, concentration and drying, respectively. The antimicrobial activities of water and ethanol extracts against bacteria were investigated. The results showed that extract of Honeysuckle's leaves has the stronger inhibition than others. The optimum extraction conditions of leaching active substances determined by the single factor experiment and the orthogonal design test method were as follows: The optimum extraction conditions of ethanol extract was obtained as the concentration of ethanol was 60%; temperature for extracting was 60℃with 4h, the ratio of material and solvent was l2:1. The optimum extraction conditions of water extract was obtained as the temperature for extracting was 70℃with 4h, the ratio of material and solvent was 14:1.The stability to temperature, acid, alkali and ultraviolet of the extract of Honeysuckle's leaves were also studied. The result showed the extract was very stable to temperature and ultraviolet. And the antibacterial activities were better when pH< 7.All extracts obtained in different fractions by using different polarities of solvents such as petroleum ether, chloroform, ethyl acetate, normal butyl alcohol and water were compared through the testing of their antibacterial activities. The results showed the antibacterial activities of ethyl acetate were strongest. To track antimicrobial components in the extracts of ethyl acetate in which many different antibacterial points appeared after diffusion. The qualitative analysis of extracting sample is conducted by the specific reaction of total flavonoids with AlCl3 and so on, It is confirmed that the extraction material is flavonoids.The extracts of ethyl acetate of Honeysuckle's leaves by polyamide columnchromatography and silica gel columnchromatography have good antibacterial activity. Antibacterial component was determined by HPLC and LC-MS. The results showed that one of antibacterial components was luteolin with a galactoside.The extract of Honeysuckle's leaves was applied in cosmetics. All the results confirmed that extract of Honeysuckle's leaves has the antimicrobial effect and the activity of antioxidation. So it can be widely used in cosmetics.