Determination Of Phthalate Acid Esters In The Food With Plastic Packaging

PEAs is widely used in plastic container and can enhance the toughness and plastic property of plastic and decrease the machining temperature. It is usually the sticky liquid or low melting point solid of high boiling point and hard-volatile, which does not react with plastic in common. Therefore, it can resolve in the alcohol or grease in the food when the plastic contacts with them. DEP, DBP, and DOP are in common use, which were used to believed harmless. However, they have been shown harm to human body as environmental hormone. In our country, DEP, DBP, and DOP have been recorded in the list of contaminating the environment, which can result in the atrophy of spermary and the decreace of sperm of rat and are poisonous to the embryo and anti-male hormone active. In order to understand the contamination extend of PEAs in the food packagings, the work takes DEP, DBP, and DOP as objects, focusing on the actuality of contamination of PEAs, studying in the following steps:1.Classifying the food with plastic sold in the market, enriching and puring with home-made Florisil-SPE, we analyse the fix quantify and nature of DEP, DBP, and DOP in the food with plastic packaging with HLPC and LC-MS. We established pretreatment method with different samples and method of determining the PEAs in food with plastic packaging, combining with HLPC, and filtering the proper solid phase extraction column fitted to determine the PAEs in the food, reviewing the effect of temperature, mobile phaseconstitution to the DEP, DBP, and DOP, optimizing the eluent under gradient and reaction time and other measuring conditions. Furthermore, we erected kinds of pretreatment methods combining with HLPC to determining the content of PAEs in food with plastic packaging. The results showed that the food with plastic packaging sold in the market are contaminated by PAEs to some extent, more than reported in the literature. The results of classic GC-MS and LC-MS shows that the outcome of the new methods is credibility and high sensitive, and the methods can be used to determine the contents of many PAEs at the same tine and fitted for quarantine department to determine the content of PAEs.2.We erected the method of determining the packaging bag of milk and transferred amount in water-solubility and grease-solubility liquor of plastic barrel of cooking oil with HLPC and the method of determining the content of DEP, DBP, and DOP in the ham by dissolve- deposition method. The water-solubility liquor, grease-solubilityliquor and n-Hexane are enriched and detected, then deposited for 5 and 10 days to determine. After that, we analyzed the difference of transferred amount of DEP, DBP, and DOP in the plastic in the food liquor by time. Dissolve- deposition method with HLPC, which takes Tetrahydrofuran as the solvent, methanol as the precipitator, can separate the DEP, DBP, and DOP from the packaging material of ham and then determine the content of them with HLPC.3.Attempting the improved LPME to determine the sample.LPME is the new pretreatment method which combines the merits of LLE and SPME and the pretreatment object needs to be a little organic extraction liquor and equipments are simple, the operations are easy with the cost is low. LPME technique is fitted for the object which is hard to resolve in the water and is novel as a pretreatment method in the water sample pretreatment technique. The method in this paper is to form the droplet on the needlepoint, which is difficult to be stable, we ameliorate the equipments of the LPME by covering the topwith a tube. Thus the droplet becomes. We enriching the DEP, DBP, and DOP in water with this method and determine the content of them with HLPC. We also revoewd the effects of different extraction liquor, extraction conditions and measuring conditions to the results.