Biodegradation Of Phenolics Endocrine Disrupting Chemicals By White-rot Fungi Pleurotaceae

Because nonylphenol and bisphenol A have teratogenic, carcinogenic, mutagenic and endocrine disrupting effects, they have been typical endocrine disrupters. Nonylphenol ethoxylates is a precursor of nonylphenol, which have been used widely as a surfactant in the industry. We simulated natural conditions, in order to inspect the biodegradation way and influence factors of bisphenol A, nonylphenol and nonylphenol ethoxylates by white rot fungi pleurotaceae.(1) Biodegradation of bisphenol A by white rot fungi in Bai Yang Dian raw water, and different effecting factors including inorganic salt medium, raw water with inorganic salt medium, bacteria coexistence, pH were investigated. The results showed that raw water of Bai Yang Dian is the optimal condition for biodegradation of bisphenol A by white rot fungi. In raw water bisphenol A was completely degraded in 6d. But the degradation is inhibited in raw water with inorganic salt medium. When bacteria coexisted, the growth of white rot fungi was inhibited. However, pH=6 was more suitable for degradation than pH=7. The intermediates during the biodegradation of bisphenol A included 2-hydroxy-2-phenyl acetic acid, 2-hydroxyphenyl propionic acid and malonic acid.(2) When bisphenol A and nonylphenol or nonylphenol ethoxylates combined pollution, we focused on the degradation of them by white rot fungi, and sodium chloride, humic acid were investigated. The results showed that when bisphenol A and nonylphenol combined pollution, the trends of bisphenol A's degradation curve is similar with bisphenol A alone, which followed the "two-stage". The degradation rate of bisphenol A is 68%, which higher than the degradation rate of nonylphenol (62%) by white rot fungi in 14 d. The addition of sodium chloride slightly promoted the biodegradation rate of bisphenol A and nonylphenol. With the concentration of humic acid become higher, the degradation of bisphenol A or nonylphenol is faster.When bisphenol A and nonylphenol ethoxylates combined pollution, the degradation rate of bisphenol A final got 72% in 14 d, and compared to nonylphenol and bisphenol A co-existence the degradation rate of bisphenol A is higher in this condition. The degradation rate of nonylphenol ethoxylates is 57%, which lower than the degradation rate of bisphenol A. The addition of sodium chloride inhibite significant the degradation of bisphenol A, with the concentration of sodium chloride increased, the degradation rate of bisphenol A significantly lower. There is little effect on the degradation of nonylphenol ethoxylates, and some parts of degradation curve are coincidence. The addition of humic acid has actively effects on the biodegradation of bisphenol A, with the concentration of humic acid improving, the degradation rate rising. Compared to humic acid-free the degradation rate of nonylphenol ethoxylates has been inhibited, but with the improvement of humic acid concentration, nonylphenol ethoxylates degradation rate increased.When bisphenol A and nonylphenol co-existence, the intermediates of bisphenol A included 2,2-two(4-hydroxyphenyl), Benzenepropanoic acid-α-hydroxy, Cinnamic acid-4-dih-ydroxy-a-hydroxy, Benzeneacetic acid-4-hydroxy, Cinnamic acid-α-hydroxy, fumaric acid and Benzoic acid-4-hydroxy. There is Benzeneacetic acid-2-hydroxy else in the condition of bisphenol A and nonylphenol ethoxylates co-existence.The intermediates of nonylphenol included Benzenemethanol-4-hydroxyl, Benzeneetha-nol-4-hydroxy and 4-hydroxyl-benzene propionate acid-2-propanol. And we detected Benzoic acid-3, 4-dihydroxy and Acetic acid-(2,4-dihy- droxyphenyl) which were the intermediates of nonylphenol ethoxylates.