Studies On Fermentation Conditions Of Chlorpyrifos-degrading Bacteria D₃

China is an expansive and agricultural country.Therefore,the production and usage of pesticide is in large quantity.The problem of rudimental pesticide pollution stands out exceptionally.The usage of pesticide has negative effects,especially the organic phosphor. Because there is not so much pertinency degradating microorganism in nature,the degradation effects are far smaller than the usage.The usage of the pesticides in large quantity leads to three "R"s(residue,resurgence,resistance),among which the rudimental of chlorpyrifos does the most harm.Strengthening the study on microorganism degradation is not only useful for taking efficient measures to eliminate pollutions,but also practical for decontaminating environment,so the study on the degradation of chlorpyrifos-degrading bacteria D₃ is done as follows.1.The optimization of the best fermentation condition for substrate,but initial C and N have the most obvious effects on microorganism fermentation.The initial C,N and pH of D₃ fermentation are studied in experiment and we get the following conclusion:Under the condition of C:N=6:4 and pH=7.0,the effect of degradation is the best.Among the three factors,the effect of C is the best,which shows that C is the key alimentation of chlorpyrifos degradation.When the proportion of C and N can't be controlled,the best single factors are as follows:the best temperature is 30℃;The best pH is 7.0;and it especially cares for oxygen.2.The mixed fermentation.Until now,the reports concerning about the microorganism fermentation of chlorpyrifos are all about single microorganism which separately reacts on chlorpyrifos and helps it implement the degradation.Two microorganism picked out are mixed together in diferent proportion to degrade chlorpyrifos and have made good effects. the best proportion of the D₃ and D₁ which this article selected is 4:1,there are many factors affecting D₃ and D₁'s mixing degradation.it is can be concluted that tempretrue effects degradation most.from this article's experiment result,Under the fermentation condition of pH is 7.0,the temperature is 30℃,the effect of degradation is the best,carbamide is The initial N.More bacteria through with the best fermentation conditions degradation of chlorpyrifos,D₃ than the degradation of chlorpyrifos alone increased 14.6 percent efficiency.3.The fun days of fermentation tank fermentation process,Fermentation culture come to the relevant parameters to further optimize the fermentation tank fermentation provided a good basis for reference,then provides the possibility for the degradation as an efficient pesticide degradation projects for industrialization of the production,this paper has a preliminary inspection of the D₃ degradation in the fermentation tank fermentation growth,Research the impact in the growth of bacteria with the fermentation tank temperature, pH,stirring to D₃ degradation,to seek the best fermentation conditions of fermentation tank fermentation process,the best single factors are as follows:the best temperature is 30℃; The best pH is 7.0;The best Mixing speeds is150r/m in Fermenter.