Research On The Technology And Reactor Of Protease Hydrolysis Assisted By Microwave Radiation

The conventional hydrolyzation reaction takes long time. This paper has mainly researched the protease-hydrolisis reaction in the assistance of the microwave radiation. The study shows that this method of microwave radiation can speed up the reaction rate.A laboratory scale microwave radiation reactor for protease-hydrolisis was designed and constructed, and the reactor consists of stirring tank or reactor, helixing pipeline reactor radiated by microwave in cavity, liquid circle system, water circle tank for the controlling the reactant liquid and so on. The over heat effect of microwave radiation can be avoided in this circulation type recator. Moreover, power of microwave radiation and the temperature of reactant liquid can be adjusted in this reactor. The stirring tank was designed and computated, The water circulation tank enables the reactant liquid in certain desireable temperature.The parameters soy protein hydrolyzed by papayin were optimized in this paper prior to microwave radiation reaction system. Firstly, the best hydrolysis conditions of papayin were studied in regular experiments, the content of nitrogen measured by formaldehyde titrated. the optimizated. craft conditions of hydrolysis analysised by the experiment of single factor and the orthogonal test are: the ratio of solid-liquid ,1:8, the contend of papayin, 5%, the temperature of hydrolysis ,50℃, the hydrolysis time, 5 h, the pH value of hydrolys ,6.7. Because the reactor is a circulating system, And the ratio of solid-liquid, 1:8, the cycle is not smooth, so the ratio of solid-liquid, 1:10. In the reactor the reaction conditions are: the ratio of solid-liquid , 1:10, the content of papain ,5%, the temperature of hydrolysis ,50℃, and the pH value of hydrolysis , 6.7, Contrast the result of 5 hour-hydrolysis experiment without microwave radiation to 120 min hydrolysis experiment under 480 W microwave radiation, the result shows that the content of nitrogen generated by 60 min-hydrolysis experiment under microwave radiation reached the content of nitrogen by 5 hour-hydrolysis experiment without microwave radiation, the rate of reaction increases more than five times. The microwave's power on the impact of hydrolysis were studied, the higher power,the sooner hydrolysis's rate.Compared the content of free amino-acid generated by 5 hour-hydrolysis experiment without microwave radiation to 60 min-hydrolysis experiment under 480W-power-microwave radiation by liquid chromatography analysis, The results showed that the content of each free-amino-acid and the total free-amino-acid are basical the same.Finally, the common nature and enlargement of the reactor were studied.The research showed that microwave-radiation can assist hydrolysis, the design of the reactor can increase the rate of action.