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Fermentation System Research Of Biodiesel Oil Production From Fibre Saccharified Liquid

Posted on:2009-10-30Degree:MasterType:Thesis
Country:ChinaCandidate:Y Y WangFull Text:PDF
GTID:2121360248456295Subject:Microbiology
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Biodiesel oil has already taken people's attention ,which is one of the most important biomass energy complement in relieving with the energy crisis.However, the major bottlenecks of biodiesel oil production is the supply of raw materials.In this paper, fermentation system to produce bio-oil have been studied useing fibre saccharified liquid.Including two main aspects, one was fermentation system optimization of bio-oil production and another was the detoxification of fibre saccharified liquid to improve the results.Therefore,it has the important theory and the practical significance.Fermentation system of Trichosporon rmentans2.1368 useing fibre saccharified liquid have been optimized. By the flask experiments of several single conditions which affect TR growth and fat synthesis,the optimal fermentation conditions were decided as follows:the added amount of 4%glucose was carbon, nitrogen is nitrogen compound of Yeast +(NH4)2SO4 which quality ratio is 3:1,C/N was 80:1,pH5.8,the amount of inoculation was 10%,Adding metal ions and 0.2%olive oil. Seal approach is sealed membrane. When TR was cultured under this circumstance,the content of fatty acids could reach 46.4 % , the biomass was 21.3 grams (dry) per liter fermentation liquid,and sugar to oil was 12.3%.The results of detoxification to the fibre saccharified liquid show that, Optimization is the added amount of activated carbon is 1.0g/L, acetaldehyde is 400mg/L, Phanerochaete chrysosporium is 10ml/L. the biomass was 21.2grams (dry) per liter fermentation liquid,and fat-transforming ratio could reach 50.9%. and sugar to oil was 13.5%.Analyzing the composition and content of bio-oil.ten fatty acids including palmic acid,oleic acid,1inoleic acid , etc were identified as the main components. which is the major components of bio-diesel..
Keywords/Search Tags:biodiesel oil, fibre raw materials, saccharified liquid, fermentation condition, detoxification
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