The Study On The Interaction Of Separation, Purification, Structure And Bioactivities Of Polysaccharides Extracted From Lentinuns With Acid

Lentinuns,widely spread in our country,is one kind of nutritious,healthy and therapeutic fungus,combining both medical and edible characteristic.It contains numerous therapeutic compounds,particular a substance,named non-immune stimulus.It has various bioactivities proved by the clinic trials,such as anti-virus,anti-tumour and declining the sugar content in the blood,etc.This research utilized the stem of lentinuns as the raw materials and the acid as the solvent,to extract the polysaccharide and identify the interaction of separation, purification and configuration.Meanwhile the research studied its bioactivity and chemical modification.The experiment included following sectors.1.Extracted the lentinuns polysaccharide with acid,setting the acid ratio, materials/acid solvent ratio,immersion time,immersion temperature and ethanol additional content as the main factors,though modular design between quadratic regression and positive rotation,reached the conclusion that the optimum process,the ratio of acid, 0.16-0.23mol/L,materials/acid solvent ratio,1:36-1:49,immersion extraction time 2.7-3.8h,immersion extraction temperature 50℃-67.8℃,and the ethanol ratio was 1:2-1:3,at 4℃,24h.The first gain ratio was 7.61%,and second time was 3.56%.2.Studyed on the method of separation and purification of lentinuns polysaccharide, and identified the concentration.The polysaccharide was rinsed by tannin acid and H₂O₂, to remove the free protein and color.After the procedures mentioned,we got the primal purified polysaccharide.Then the sample was rinsed by gradient ethanol,and three different level polysaccharide were obtained,named SP₁,SP₂,SP₃,25.58%,64.77%,9.64%, respectively.The SP₂ whose content of Uronic acid and protein was 28.11%,1.20%.This portion was sole purified polysaccharide after it was identified though the method of frozen centrifugation,Sephadex G-100 Gel Permeation chromatography.3.Detected the configuration of SP₂ primally.The molecular weight of SP₂,average 5.203×10⁴Da,was deterred by GPC.After the GC examined the composition of SP₂,we could find that SP₂ contained several monosaccharide,and major portion was glucose,others wasMannose and Galactose.The infrared scanning results demonstrated that SP₂,configuration was typical D-Glucopyranosyl.4.Inspected the capability of anti-oxidation though the method of chemical radiation,and the results demonstrated that SP and SP₂ had the capability of scavenging O₂-·,radical OH·,H₂O₂.It showed that SP and SP₂ had the capability of inhibiting the hemolysis of mouse's red cell and forming of MDA located in the mice's liver's slurry and swelling degree of Mitochondria in the liver,by the means of stimulating In vitro systems by chemical approach,and anti-oxidization reaction in vivo.