Selecting And Culturing Of Bacteria Used To Degrade Oil And Pilot Study Of Bio-Remediation

Three oil-resistant strains were identified.They were separated by culturing, domesticating and selecting form the contaminated soil of Liaohe oil field. The separated strains were proven that they have good degradation ability to the crud oil by fluid-phase decomposition tests. In this study, the culture medium as well as the shake flask operation condition, namely the optimum culture temperature and pH, was optimized by Orthogonal Tests. The growth models were also constructed according to the growth curves of the separated strains. A bacterium agent which was made using the three bacteria was added to the oil contaminated soil located in Liaohe oil field. This pilot test indicated that the oil content of the contaminated soil was distinctly reduced about 70% by biodegradation in 60 days. Meanwhile the growing pattern of the predominant bacterium group was obtained based on the amount analysis of the microbo. The contact of the bacteria and the oil-contaminated soil is a necessary precondition of soil remediation, so the transportation law of bacteria was also studied in this paper. The parameter of soil pores was obtained based on the soil granule structure characteristic and fractal theory, then the bacteria diffusion coefficient was obtained by utilizing the membrane cell. The equilibrium absorbtion model of the bacteria in soil was identified by thermodynamics balance experiment, which was the bedrock of the constructed microbo transportation model. By analysing the transportation parameters, contribution of each parameter to the transporttion was dug out, and the understanding transportation law of microbio was deepened.