Study On Desulfurated Bacteria H-412 Immobilized And Desulfurating Performance

Biodesulfurization (BDS) is a newly high developing and environmentally benign biotechnology. BDS, in which anaerobic bacteria or aerobic bacteria are used in the selective removal of sulfur from petroleum and its distillates, is C-S-band-targeted but C-C-band-targeted fashion so that it can keep the caloric value of fuel. BDS technology has many advantages, such as easy manipulation, low-SO2 emission and energy consumption, low cost and so on.Biodesulfurization is hotspot in actual study, immobilized method can solve the problem that dissociative bacteria faced, such as separate oil-water difficult, bacteria reuse and lifespan short, the ability of bear acid,alkali and impact bad, etc. But the key in this technology is select carrier and method.The author study on biodesulfurization used immobilized bacteria H-412. The optimal immobilization desulfurization bacteria H-412 conditions of sodium alginate, polyvinylalcohol and SiO2 were obtained. The suit conditions of immobilization are as follows: The experiment results indicated that the immobilized cell had the optimum desulfurization effect under the conditions of 4oC saturated boracic acid including 1 % CaCl2 as cross-linking agent, 3 % SA, 4% PVA and 0.5 % diatomite as carrier, the content bacteria is 5%.The desulfurating performance of immobilized bacteria H-412 is better than free bacteria in oil desulfurization. When ratio of oil to water is up to 2/3, after 48h reaction, the degradation rate of immobilized bacteria H-412 remains above 60%, but degradation rate of bacteria low 20%. The immobilized bacteria H-412 can repeatedly use several times, after seven times circulation the immobilized bacteria remain has 65% desulfurization ratio. Compaer whit free bacteria, immobilized bacteria have low sensitivity and hight stability.