Study On Effects Of Nitrobenzene In Water On Edible Safety Of Fishes

In this paper, various concentrations accumulations of nitrobenzene by spinal muscle and internal organs in fish bodies have been investigated in water through the laboratory simulation. The results shown that when the average concentration of nitrobenzene is 0.586 mg/l, the average content in chiropractors muscle in fish bodies is 1.34mg/kg, and the accumulation factor is 2.29; when the average concentration of nitrobenzene is 0.014mg/l, the average content in spinal muscle in fish bodies is 0.048mg/kg, and the accumulation factor is 3.6. On this basis, the metabolism course of nitrobenzene in fish liver has been studied and the metabolites have been identification under both in-vitro and in-vivo laboratory conditions. The in-vitro experimental study showed that the nitrobenzene content in fish liver decreased gradually over time. In this progress, reduced nitrobenzene is the main metabolism process, and the content gradually increasing with the generation of the metabolites-aniline; the in-vivo experimental study shows that the accumulation curve of nitrobenzene in fish liver appears peak shape, which is the same as the accumulation curves in high concentration group and low concentration group. Moreover, when the concentration of nitrobenzene decreased, the content of metabolite-aniline has an increasing trend, especially after 7-10 hours.According to evaluation procedures of food safety, the edible safety quantity in fish is estimated to be 0.030mg/kg. This value can be taken as the fish edibles safety evaluation basis of Songhua River nitrobenzene pollution. The water pollution accident will influence the fish edible safety in Songhua River directly, and the nitrobenzene is the primary pollution factory and the aniline is the minor one. The identification on the metabolites needs further confirm using LC/MS method.