Toxicokinetics And Toxicodynamics For Benzo[a]pyrene In Chlamys Farreri

Recently, because of development of maritime petroleum, marine traffic and industry sewerage discharge, polycyclic aromatic hydrocarbon (PAHs) pollution became seriously. 16 kinds of PAHs became priority organic pollutants. Benzo[a]pyrene (BaP) is one of the most carcinogenic PAHs. Chlamys farreri is important economy bivalve cultured bu float valve in northern coast of China. We adopted laboratory study and sea area sampling, study the toxicokinetics and toxicodynamic of BaP in C. farrery,and a physiologically based toxicokinetic and toxicodynamic(PBTK-TD)model was developed for BaP in scallop (Chlamys farreri).What can be used in security of aquatic food, protection of halobios and oceanic environment.1. Research on bioaccumulation and elimination of benzo[a]pyrene in tissues of scallop (Chlamys farreri)In the experiment, 4 treatments were set up to investigate bioaccumulation and elimination of benzo[a]pyrene (BaP) in tissues (including soamtic tissue, gill,haemolymph, digestive gland and adductor muscle) of scallop (Chlamys farreri). The results suggested that BaP exposure had significant effects on BaP content in tissues of scallop (P<0.05). In haemolymph, there were no obvious difference of B[a]P content among all treatments (P<0.05). BaP content increased rapidly at the beginning of exposure and maintained at a high level during the experiment. After exposure cessation, B[a]P content in haemolymph descended appreciably at the late elimination experiment only in 0.05μg/L treatment. While BaP content in other tissues was affected markedly by BaP concentration of exposure water (P<0.05). There were marked difference of BaP content among the same tissue in different treatments, and the same situation were seen in different tissues treated with the same treatment. The change of BaP content of each tissue in all treatments exposure showed two peaks, that was at the beginning of exposure, BaP content in tissues rised rapidly, and then descended appreciably, but increased afterwards. At the initiation of elimination experiment, BaP content in tissues dropped swiftly and then became stabilization, which stayed at a high level at the end of the whole experiment. The elimination rate of BaP in all tissues showed negative relation with the lipid content in corresponding tissues. What can be concluded is that the ability of bioaccumulation of BaP in scallop is mighty and the cumulative content shows time response.2. A physiologically based toxicokinetic model for benzo[a]pyrene in Chlamys farreriThe Physiologically Based Toxicokinetic Model for Benzo [a] Pyrene (BaP) in Chlamys farreri physiological characteristic of C. farreri and transfer rule of BaP. This article mainly research about the toxicokinetics of BaP in somatic tissue , gill , digstive gland and adductor muscle : There are lots of deals present themselves as One-compartment Model , such as somatic tissue , gill and adductor muscle dealed with 0.05μg/L and 0.5μg/L , others are accord with Two-compartment Modle ; They are tally with Two-compartment Modle during the eliminate period . We can conclude from this experiment that BaP have the ability of accumulate in Chlamys farreri quickly , but the half life of elimination is to long , and the rate of metabolize is comparatively slow . The parameters of poison metabolize conclude from this experiment can forecast the distribution of BaP in Chlamys farreri and the change rule of content in different tissue , so the concise PBTK model came into being .3. A Toxicokinetic and toxicodynamic models for benzo[a]pyrene in Chlamys farreri Based on PBTK model for BaP in, this study we import toxicological index in gill and digestive gland: included AHH, MDA, single cell gel electrophoresis (SCGE) assay and oxidative carbonyl protein, a physiologically based toxicokinetic and toxicodynami(cPBTK-TD)model was developed for BaP in scallop (Chlamys farreri). We found that, BaP content and toxicological index in gill and digestive gland showed time and dose correlativity, accorded with cubic equation. What can be conclude is that the toxicological index in gill and digestive gland of scallop can reflect the BaP content in scallop tissue, whereas the component of BaP in tissue can direct the damage degree. It is very important for security of aquatic food, protection of halobios and sea environment.4. Bioaccumulation and toxicological effects of benzo[a]prrene in Chlamys farreri from offing of QingdaoSub-surface seawater and scallop (Chlamys farreri) samples at 3 sites(include Honsshiya, Taipingjiao and No.8 dock) in the sea area of the Qingdao in Apr. 30 and Jul. 30 of 2007, Benzo[a]Pyrene (BaP) content of seawater and tissue of scallop, toxicological index of gill and digestive gland were analyzed. As a result, the BaP in seawater is NO.8 dock > Taipingjiao > Hongshiya(P<0.05), in individual site April < July. Compare content of BaP in seawater and scallop tissue, a good correlation is observed between sea water and partial scallop tissue(gill and somatic tissue) (P<0.05), there is no significant difference between other tissue of scallop sampled from different sea areas.The frequencies of comet cells and oxidative carbonyl protein content in scallop tissue (gill and smatic tissue) were obtained as follows, No.8 dock > Taipingjiao > Hongshiya(P<0.05), AHH activity and MDA content had no significant difference, except the gill of scallop simpled from No.8 dock is higher than others.