Isolation Of Halophilic (Halotolerant) Microorganisms And Its Application To Pickled Vegetable Processing Wastewater Treatment

A lot of studies have shown that halophilic/halotolerant microorganisms extensively distribute to diverse hypersaline environments in the world, such as salt lakes, hypersaline soils, marine environments, etc. The property of microbiol adaptation to high salt environments is mainly related with their enzymes and other bio-active substances. Much interest in halophilic adaptation has been supported by the studies for the origin of life and the application to industrial manufacturing, food processing and contaminated environment bioremediation. So it is very significant not only in the theory but also in practice to study halophilic/halotolerant microorganisms.The author briefly reviewed the classification, distribution and adaptative mechanism of halophilic/halotolerant microorganisms, described the advance of saline wastewater treatment and the application of those organisms to the treatment.We have isolated diverse halophilic or halotolerant strains from various environmental samples using different media. The analysis of 16S rDNA sequences of the isolates showed that there might be three novel species. After the domestication in the low pH, high salt and organic content pikled vegetable processing wastewater, a flora of high COD removal efficiency microorganisms which can also adjust the acid wastewater to neutral was obtained. Later, the culture was reinoculated to the wastewater, when the HRT equals 2 days, the COD removal efficiency reached 97% without any physical-chemical pre-treatment. This work suggested a practical strategy to saline wastewater treatment.