A Study Of Effect And Molecular Mechanism On Male Mouse Sertoli Cells Induced By Environmental Estrogen

The environment estrogen(also called endocrine disruptors,EDs) refers the substance which exists in the environment,the substance can disturb the person or the animal secretion systerm and causes the unusual effect. Damage to the living environment of human beings are increasingly becoming a serious problem facing humanity. It affects the biological nature,including human health and lead to diseases.The influence of EDs is various;especially the harm of the male reproductive system.However,its functionary mechanism is extremely complex,especially the molecular mechanism is not known at present. And environment estrogen also has synergy,individual detect a certain kind of environment estrogen activity may underestimate their potential harmfulness.This is even more increased its endanger and the complexity of the mechanism,So it is necessary to research the effect of some common environment estrogen mixed.Using the combination enzyme digestion,selective adhesion method We gained high purity Sertoli cells in mice; The different density (10-9,10-7,10-5mol/L)BPA,Soybean isoflavones,the combined of BPA and Soybean isoflavones,environment estrogen and testosterone mixed were joined into the DMEM culture medium in vitro to induce Sertoli cells. We used laser confocal microscope to detect the expression of cytochrome C and expression of Bcl-2 families'proteins.The results:1. Using the combination enzyme digestion,selective adhesion method,the purity of sertoli cells reached 90% higher2. Confocal microscope detection experiments showed that: Compared with control group, BPA or Soybean isoflavones in the low dosage(10-9mol/L) induced.The expression signal of Cytochrome C was increasd, in Bcl-2 families'proteins, The expression signal of Bax was weak, and The expression signal of Bcl-2 was increasd; With the increasing concentration of BPA,Soybean isoflavones, the signal of Cytochrome C decreased gradually, in Bcl-2 families'proteins , the signal of Bax gradually increased,the signal of Bcl-2 decreased gradually,demonstrating that the Sertoli cell proliferation activity may be activated by the low concentrations of BPA or Soybean isoflavones,while the apoptosis of these cells was stimulated the high concentration of the agents.3. In the BPA and Soybean isoflavones co-treated group, the low-dose of adiministration(10-9 mol/L) caused,the reduction of Cytochrome C protein expresses Weak, in Bcl-2 family proteins, the expression signal of Bax is Weak,the expression signal of Bcl-2 is strong; However,after the co-treatment of both agents at higher-doses(10-5mol/L), the expression signal of Cytochrome C was markedly increasd, in Bcl-2 family proteins, the expression signal of Bax was markedly decreasd, the expression signal of Bcl-2 was markedly increasd, suggesting an overlay effect of BPA with Soybean isoflavones at low dose, and an antagonistic effect at higher-dose.4. In the environment estrogen and testosterone co-treated group (10-5mol/L), compared with separately, the expression signal of Cytochrome C markedly improved, in Bcl-2 families'proteins, the expression signal of Bax is clearly decreasd,the expression signal of Bcl-2 markedly improved, and the environment estrogen and testosterone appear antagonistic phenomena.These results displayed that, The signal role of BPA or Soybean isoflavones to mouse Sertoli cells may be the same; A n overlay effect of BPA and Soybean isoflavones may occur when both agents were treated at low dosage,while the treatment at high dosage may cause an antagonistic mechanism.Furthermore,EDs can also act via its antagonistic roles against androgen.All the effects of these agents on the growth and apoptosis in Sertoli cells may mediat by mitochondrial pathways,in which Bcl-2 families'proteins are involved.