Studies On The Inspection Methods And The Antioxidaive Effect Of Effective Components From Zanthoxylum L.

Chinese prickly ash is ripe dry pericarp of Zanthoxylum Bungeanum Maxim or Zanthoxylum schinifolium Sieb.et Zucc,which belongs to the kind of Rutaceae Zanthoxylum L.Zanthoxylum has been applied for two thousand years in our country as main condiment and traditional Chinese medicine dosage.The chemical compounds are alkaloids,alkylamides,neolignans,volatile oil, coumarin and so on.Alkylamides are main numb-taste components and volatile oil is main aroma components.All of the components in Zanthoxylum.have special functions.Used the fruits of Z Schinifolium sieb.et zucc as materials,the numb-taste components were isolated and extracted by the means of a series modern technology such as modern isolation and extraction technology and instrument analysis technology.Compared to the purification of numb-taste components made by myself,the quantities of numb-taste components from fruits of Zanthorylum bungenum,oil,were inspected by HPLC,near infrared spectroscopy and ultraviolet spectrometer.The result indicated as follows:1.Compared to standard sample made by myself,the ultraviolet spectrometer method was built up to test the quantities of numb-taste components.The average rate of recovery is 76.4%—84.5%,the efficient of variation of repeatability test is 0.20%-0.91%,the limit of detection is 0.0485μg/mL.2.Compared to standard sample made by myself,the HPLC method was built up to test he quantities of numb-taste components.The average rate of recovery is 86.4%—112.1%;the efficient of variation of repeatability test is 0.72%-4.85%;the limit of detection is 0.0339μg/mL.The method is reliable and accruate.3.Using partial least squares(PLS)regression,the models were built up by the same sample set of 118 samples in 9 kinds of acquisition parameters with the resolution(4cm^-1,8cm^-1, 16cm^-1)and the sample scans(32,64 and 128).Experiments demonstrated that the model at resolution of 16cm^-1]and sample scans of 128 was better than the others,and the determination coefficient and RMSECV of cross validation were 0.907 and 0.509.The results show that NIRS can be used as a method to rapidly and nondestructively predict volatile oil content in intact Zanthoxylum bungeagum Maxim.4.By inspecting the colour of 141 samples of Zanthoxylum L,the result indicated that they are related between A^*,L^*and place of production.However,the relation isn't inevitable,the colour of Zanthoxylum L from different place has his own strong points,so it deserves the next research.5.Studies on the antioxidaive effect of effective components from Zanthoxylum L.The result indicated that the combination of lard,soybean oil and the additive is antioxidaive,lard+ numb-taste components+Vc and soybean oil + numb-taste components+citric acid have the best effect of antioxidation and obvious coordination.The antioxidation of adding singly the numb-taste components and oil from Zanthoxylum L is better han the mixture of them.