Study On The Ultra-high Pressure Processing Of Pueraria Lobata Beverage

Pueraria lobata has been widely used and planted as an important medicinal and edible value material. With the development of modern medical technology, research shows that isoflavone and puerarin in Pueraria lobata have more function, such as anticancer, control cardio-cerebrovascular disease, anti-aging, antialcoholism, etc. A new method is exigent to improve and development the past processing disadvantages.The traditional processing methods of antialcoholism beverage, the hot immersion method, the decocting method and high-pressure steam sterilization, have many disadvantages such as complex process, long extraction time and afflictive color and taste. Isoflavones can be extracted from Pueraria lobata by ultra-high pressure extraction (UHPE) and ultra-high pressure technology (UHPT) has also sterilizing effect. According to color, taste and aroma study, the most suitable process which antialcoholism beverage is high isoflavones content, also adding Pueraria lobata flower, Hawkthorn and hovenia dulcis thunb.The key experiments in the dissertation are as follows:1. Applying UHPE technology to extract isoflavones from Pueraria lobata. It was investigated that the influence of ethanol concentration, extraction pressure, immersion time, liquid-solid ratio (v/w) and extraction times for extraction rate. Finally, the optimum extraction conditions of isoflavones from the Pueraria lobata by UHPE are water extraction; extraction pressure, 300 MPa; liquid-solid ratio (v/w), 60:1; extraction time, instantaneous extraction; immersion time, 0 h; extraction one time. The yield of total isoflavones is 73.6020 mg/g. Heat reflux, hot immersion extraction and cold immersion were also used to extract isoflavones. For the extraction time, UHPE was shorter than other four methods.2. Adding flowers of Pueraria lobata, seeds of Hovenia dulcis Thunb and hawkthorn into beverage. Immersion extraction was used as the contrast methods. The extraction yield of isoflavones and flavonoids from the flowers of Pueraria lobata, Hawthorns and seeds of Hovenia Dulcis Thunb were all higher than immersion extraction. The extraction time of UHPE is shorter than other four methods. Thereby, UHPE is the optimal extraction method.3. Adding Acesulfame-k as sugar substitute. The final beverage formula: Pueraria lobata: hawkthorn: flowers of Pueraria lobata: Seeds of Hovenia dulcis Thunb = 1:1:0.5:0.5:0.1.4. The antioxidant activity was tested in this paper. DPPH, Hydroxyl radical and liposome oxidation methods were applied to test the antioxidant activity of the Pueraria lobata extract and the beverage. The antioxidant activity of beverage was higher than Pueraria lobata extract. And the antioxidant activity of Pueraria lobata extract and the beverage with concentration above 0.5 mg/mL was as high as 0.1 mg/mL VC.5. Through studying on antialcoholism beverage, we can know that it can reduce the drunk time of the mice into which filled the antialcoholism beverage and after drunk than blank control. The results show that this beverage has both antialcoholism function and preventing alcoholism function. The antialcoholism function and preventing drunken function of beverage are related to dosage. The preventing alcoholism function was significant.Filling the antialcoholism beverage into the mice, after being drunk, the ethanol concentration in blood of mice which be filled are fewer than blank control minutes. It shows that the extract of antialcoholism beverage could reduce the ethanol concentration in mice blood.6. Test some physical and chemical properties of the beverage, we can know that: the total number of bacteria is 0; dissolved solid material in the beverage is 9.32%; acid content is 1.8%; protein content is 25.8 mg/L.