Research On Biodegradation Technology Of Chlorinated Organic Compounds

The epoxy propane wastewater contains high concentration of chlorinated organic compounds, such as chloropropanol, propylene glycol, dichloroisopropy-lether and chloroacetone etc, which is the rub of the biodegradation treatment. Using epoxy propane wastewater as a starting point, the main research contents include three aspects: the adaptable mechanism research of existing usual bacterium for the epoxy propane wastewater, the biodegradation study of 1,3-dicloro-2-propanol structural resembling chloropropanol in the field of biology, and the clone and expression research of the key enzyme of goal gene in the biodegradation pathway by molecular biology.Firstly, it is the adaptable mechanism research of existing usual bacterium in lab for the epoxy propane wastewater. Halophytes bacilli J7, Escherichia coli DH5αand bacteria WH-ZJ were inoculated respectively to the culture mediums, which nutrients, pH and concentration of Cl~- were different, and then investigated the growth of three bacteria by vibrating cultivation. The results of the experiment are as follows: after salt-tolerant domestication, the bacteria WH-ZJ, which adapted the epoxy propane wastewater with 18000mg/L concentration of Cl~-, can adapt the epoxy propane wastewater with 32000mg/L concentration of Cl~-, which means the high salinity is not the obstacle to restricting the growth of bacteria. The bacteria can not make use of the organic compounds in the epoxy propane wastewater, effectively. Therefore, the chief ingredient in the restricting growth of bacteria is the biodegradation ability to the organic compounds consisting in the epoxy propane wastewater.Secondly, by using bioinformatics in the field of biology, the biodegradation mechanism of 1,3-dicloro-2-propanol, a structural similar with chloropropanol the main ingredient of epoxy propane wastewater was investigated. The relevant information and the sequence information of DNA of halohydrin epoxidase A and halohydrin epoxidase B, which are the key enzymes in the process of the biodegradation of 1,3-Dichloro-2-propanol, were acquired. And the homology analysis of halohydrin epoxidase A and halohydrin epoxidase B were made. The result shows that relevant microbes that can synthesize those key enzymes are widely spread in the nature. And the typical relevant microbes are as follows: Corynebacterium sp., Arthrobacter sp., Agrobacterium-tumefaciens, Burkholderia sp., Mycobacterium, Chloroflexus aggregans and Heliobacillus mobilis etc.Finally, by using the method of molecular biology, the Corynebacterium sp. AB91054 was chose to be the testing bacterium. And the results are as follows: extracted the genome DNA of Corynebacterium sp. AB91054, amplified the hheB goal gene by the PCR technique.