Research On Chemical Composition In Dairy Fan And Identification Of Lactic Acid Bacteria From Acid Whey In Yunnan

Microbiological composition of 20 samples of acid whey and chemical composition of 16 samples of dairy fan in Yunnan Dali the Bai nationality Region were determined. The average contents of dry-matter, fat, protein, lactose, ash, calcium and phosphorus were 87.65±0.33g/10~0g, 46.00±0.43g/10~0g, 33.34±0.25g/10~0g, 2.64±0.04g/10~0g, 593.51±0.07mg/10~0g and 467.37±0.03mg/10~0g respectively.Microbiological composition of the 20 samples of acid whey was lactic acid bacteria. The counts of lactic acid bacteria in 3 samples were from 6.00×10~4 cfu/mL to 8.50×10~4cfu/mL, 5 samples were from 2.83×10~5cfu/mL to 8.36×10~5 cfu/mL,7 samples were from 1.40×10~6cfu/mL to 5.91×10~6cfu/mL, 4 samples were from 1.16×10~7cfu/mL to 8.41×10~7cfu/mL, 1 sample was 1.59×10~8 cfu/mL.According to their morphological, physiological and biochemical properties, including chemical characteristics, among 85 lactobacillus, 73 lactobacillus were identified to 16 homofermentative species or subspecies of lactobacillus, and 12 lactobacillus were identified to 5 heterofermentative species of lactobacillus among the homofermentative lactobacillus, L. acidophilus (26 strains), L. gasseri (10~ strains), L. crispatus (9 strains), L. curvatus (5 strains), L. helveticus (5 strains), L. delbrueckii subsp.lactis (3 strains), L. amylophilus (2 strains), L. johnsonii (2 strains), L. plantarum (4 strains), L. jensenii (1 strains), L. alimentarius (1 strains), L. coryniformis subsp. torquens (1 strains), L. coryniformis subsp. coryniformis (1 strains), L. casei subsp.pseudoplantarum (1 strains), L. sake (1 strains), L. casei subsp. casei (1 strains). Among the heterofermentative lactobacillus, L. fermentum (6 strains), L. fructivorans (2 strains), L. parabuchneri (2 strains), L. brevis (1 strains), L. sanfrancisco (1 strains).9 cocci were identified to 3 categories and 4 species, 2 cocci were identified to Leuconostoc, 4 cocci were identified to Enterococcus, 3 cocci were identified to Streptococcus. 2 cocci of Leuconostoc were identified to Leuc. Lactis, 4 cocci of Enterococcus were identified to Enterococcus. Faecalis, Among 3 cocci of Streptococcus, S.agalactiae (2 strains), S.sanguis (2 strains).Among the lactobacillus,3 strains could grow at pH3.0, 15 strains could grow at pH3.5 and others neither grow at pH3.0 nor pH3.5.