Extraction And Purification Research Of D-chiro-inositol From Buckwheat

Buckwheat which belongs to the Polygonaceace family adapts to cool, moist climates and short growingseason. Plant taxonomy mainly has two varities of Fagopyrum tataricum Gaert and Fagopyrum esculentumMoench. Buckwheat contains high biological value protein, vitamin, mineral, flavanoid and so on.Buckwheat contains many biological functions, such as degrade blood sugar, degrade blood fat, anticancer,antioxygen and eliminate free radical. Research discovers that buckwheat seed contains little DCImonomer and lots of DCI derivate- Fagopyritol. It can effectually regulate blood sugar and amelioratediabetic (especiallyⅡtype diabetic) symptom.DCI is the stereo-isomer of inositol, as a new generation insuline acceptor which can effectuallypromote insulin function, degrade blood sugar, triglyceride level and so on. Further reserch discovers: DCIhas an important effect on the unique single conduction process of degrading blood sugar hormone-insulin.When vivo defects enough DCI, it can result in insulin resistance phenomenon. Therefore supplying withDCI can improve organism soma to insulin sensitivity and eliminate insuline resistance. Fundamental DCIaccommodates organism physiological functions and metabolic balance, so it degrades diabetic incidence.In this study, adopting three kinds of extraction methods for researching extraction technology of DCI,methods include different solvents extraction method, microwave method and supersonic wave method.Research technology conditions with different extraction methods and definite optimal technologycondition. Experimental result indicates, when adopting different solvents extract DCI from buckwheat,solvent as alcohol has the best effect. The optimal technology condition: temperature 30℃, alcoholconcentration 50%, solid-liquid ratiol:20, time 1.5h, progress second order extraction, the highest yield ofDCI is 4.95mg/g; When microwave method extract DCI from buckwheeat, mono-factor and orthogonalexperiment that determine the optimal technology condition: microwave power 245W, microwavewarm-up time 120s, alcohol concentration 80%, solid-liquid ratio 1:30, the DCI content achieves5.09mg/g; When adopting supersonic wave method extract, the optimal technology condition, alcoholconcention 50%, solid-liquid ratio 1:15, extraction time 30min, extraction temperature 50℃, the DCIcontent achieves 5.19mg/g. Three different extraction methods is compared with influence to DCIextraction. Then determine the best effect for extracting DCI with supersonic method.At the base of extracting crudely DCI from buckwheat, in order to improve DCI content in buckwheatextraction and reduce soluble carbohydrate and other impurities. The research adopts two differentmethods for buckwheat extraction.The first method adopts different kind microbes to ferment buckwheatextraction. The method can increase DCI and its derivation content in buckwheat seed solution, while itreduces sugar content in extraction. The research adopts Saccharomyces cerevisia, Mucor, Bacterium coli, Torulopsis apicola, Aspergillus oryzae Bacillus subtilis, Candida mycoderma, Black mold that inoculate inbuckwheat extraction. Determine content change of various compotent with tracing. Culturing withAspergillus oryzae for five days, the DCI content in buckwheat extraction that increases to 6.07mg/g, whilesugar content decrease 53%. It is the fittest condition of extraction composition for synthetic consideration.Adopting activated carbon column chromatography to purify sample, DCI and some sugar alcoholmaterials can adsorb to activated carbon column, then using gradient elution. Sample determines withHPLC, DCI content achieve 31.8%. The method can simply DCI techology of purifying, reduce solventdosage in purified process and degrade production cost.The second method adopts sprout and acid hydrolysis method to improve DCI content in buckwheat,mostly DCI exists with derivation pattern. With sprouting buckwheat seed, DCI monomer contentincreased obviously, as eight times as before. Therefore the research uses sprouting buckwheat seed ascontinual experimental material. Acid hydrolysis causes DCI derivation invertion to DCI, obviouslyelevates DCI content in buckwheat: Meanwhile mostly carbohydrate invert to insoluble by-product, andmake simply for DCI purity procession. Experimental result indicates, the optimal technology of DCI: HClconcention 9N, temperature 95℃, time 18h, solid-liquid ratio 1: 10. At this condition, the DCI content ofextraction arrive to 62.5mg/g, about fifteen times with nonsproutand acid hydrolysis. Purify procession ofextraction that dissolves with same volume, adds 95% alcohol of two times volume, stirs 1 hour forsufficient reaction at 70℃, cools to 15℃, and gets DCI precipitate. The DCI purity of precipitate is lower,so the process that dissolves the precipitate and adds alcohol solution. Repetat heat and cool treatment,progress of recrystallization. After several recrystal treatments, the DCI content achieves 432.92mg/g.