Research On Chinese Alcohol By Gas Chromatographic/Mass Spectrometric Analysis Of Nonvolatile Compounds

There are hundreds of kinds of compounds in Chinese alcohols, the contents ofwhich decide the tastes of different kinds alcohols. These compounds can be divided intovolatile and nonvolatile. The volatile compounds were wildly studied in the past,Comparatively few papers about the nonvolatile were reported. This has affected people'sunderstanding to Chinese alcohol.The nonvolatile compounds in different Chinese alcohols were compared by gaschromatographic/mass spectrometric (GC/MS) after derivatized with BSTFA(N,O-bis(trimethylsilyl) trifluoroacetamide) + TMCS (trimethylchiorosilane) reagent.The main work were listed below.(1) Different pretreatments were reviewed and compared, in which freeze dry systemwere choosed as the pretreatment for qualitative analysis and at the stage of trying themethod of chromatography; The method of water bath with reduced pressure were usedwhen quantitative analysis.(2) The optimal condition of derivatization was choosed after studying the effect ofdifferent compounds when they were derivatized at different temperatures and differenttime. And the results showed that heated with water bath at 70℃for 40min was theappropriate condition.(3) Several typical Chinese alcohols were compared, such as the Moutai spirit,Jiannanchun spirit, Fen spirit, and Guojiao1573. The results showed that the contents ofthe nonvolatitle in different alcohols were very different: the contents of thenonvolatitle in Moutai were the highest, Guojiao1573 were the second ,Jiannanchunspirit were the third, and Fen spirit were the lowest; There were high contents of fattyacid ether ethyl and lactic acid in Moutai spirit, So did Jiannanchun spirit andGuojiao1573 ,but the contents in Moutai spirit were higher than others, and there was ahigh content of sugar in Jiannanchun spirit and Guojiao1573,which was up to80.36mg/100mL.