Preparation And Immunoassay Of Dye And Compound Doped Fluorescent Nanoparticles

Recently, fluorescent doped nanoparticles have become an attractive area of research. As a novel fluorescence material, the nanoprticles have advantages of strong fluorescence, high stability and good biological compatibility compared to conventional organic fluorescence probes. Thus they can be used as luminescence probes extensively in biological detection and diagnosis.On the first of this paper, a monomer silica precursor was obtained by coupling fluorescein 6-AFM with DTPAA and APTEOS, a fluorescein doped silica nanoparticles with litter dye leakage was prepared. Then integrated with nanotechnology, biotechnology and fluorescence labeling techniques. A fluoroimmunoassay for detecting trace level human IgG was developed, The calibration graph for human IgG was linear over the range of 0.1-225ng/ml with a detection limit of 0.06 ng/ml.However, fluorescein of phenylporphyrin also fluorescent leakage when captured in silica nanoparticles, because of fluorescence quenching in water solution. Based on it, our work was attempt to use polyacrylonitrile/ Polystyrene nanoparticles for enentrapping dye of phenylporphyrin. Above them, Polystyrene nanoparticles can limit fluorescence quenching in solution. However, Polystyrene is highly hydrophobic, the method was confine to further application for it may easily aggregation in solution.This paper combines characteristics of the above two types nanoparticles, prepared Submicron Polystyrene/Silica hybrid Particles with the entrapment of fluorescein of phenylporphyrin. This hybrid Particles behave highly hydrophilic like silica oxides, can easily disperse in solution, also the water solution cannot enter into the hole of nanosphere because of the hydrophobic of Polystyrene. The particles were found to be good carriers for entrapped dye molecules with no leaching or quenching in water solution.