| Alkylphen01s (APs) are stable in environment and have been known as endocrine disruptors to organisms. In order to GC-MS determine APs from biota samples containing large amount of lipids, base on previous studies on water, particulate and sediment, a simple and economic cleanup technique was developed and it was applied in Tumen River Environmental samples. The technique was combining florisil adsorption chromatography and silyl derivatization technique. The low and high (non-polar and high polar) molecular lipids were removed from the biota samples with deactivated florisil column and middle molecular (middle polar) lipids were removed with activated florisil column after the APs were converted to corresponding silyl derivatives with bis (trimethylsilyl) trifluoroacetamide (BSTFA). On the basis of the above results, a simple cleanup treatment kit was developed to improve the convenience. The recoveries of APs from spiked biota samples varied from 88 to 103% with a low relative standard deviation (mean value: 5.3%). The technique was successfully applied to fish samples from Tumen River. Tumen River is an important basin in Yanbian state of Jilin province. To characteristics of APS in Tumen River, dissolved water, particulate, surface sediment and fish samples were collected from 12 stations inApril, August, October 2006 and March 2007 and it were analyzed with GC-MS. Among the 7 APs determined, nonylphenols (NPs) were predominant pollutant. The NPs concentrations ranged from 11.7 to 3009.8 ng L-1, low of detection limit (LOD) to 884.8 ng L-1, LOD to 2784.2 ng g1 dw and 11.6 to 47.0 ng g-1 dw in dissolved water, particulate, sediment and fish samples, respectively. Levels of NPs were showed seasonal variation and decreasing trend with far from the suspected pollution sources. In addition, bioconcentration factors (BCFs) in fishes were 12.3-49.7. Levels of NPs in Tumen River may cause to the undesirable biological effects. |
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