The Extraction And Purification Of Andrographis Paniculta

Andrographis Paniculta is the normal Chinese herbal medicinen quantities ofmedical researches show that Andrographis Paniculta lactone,the ingredient inAndrographis Paniculta has the function of anti-inflammation, bacterial, virus, anddiseases of Cardiovascular.This thesis takes ethyl alcohol as extraction solvent and the extraction ratio ofandrographolide as assessing standard, thus the comparatively good craft conditionshould be: when the temprerature is 50℃,pH=4,add 1300U Cellulase into every 50gandrographis Paniculta,and get processed for 100 minute;then add ethylalcohol(75%), 10 times volum of the raw stuff; the extraction ratio of andrographolideattains 98%,the yield without cellulase is 7.43%, and the yield of solid with cellulaseis 8.91%,which is not obviously more than the last one.The big hole resin DA201-B is the best resin for purification of andrographispaniculta lactone is thus assured. Finally, the best absorption craft conditon isdetermined: the flow volecity of raw solution is 1BV/h,and do not change the pH ofthe raw solution.the solution which resin can deal with is 21 times than itself, and theabsorption amount is 13.42mg/ml resin,and the yield rate is 90.25%.And the bestdesorption condition is:60%,pH=5 alcohol,which flow volecity is 3BV/h,in the bestdesorption condition, andrographolide desorption rate is 84.95%. all the absoptionand desorption are on normal temperature.It is showed by the number of theexperiment that on the best condition of the experiment craft,the purity ofandrographis changed from 24.984/0 to 74%,and the yield of solid changed from8.91% to 3%.The retain rate of andrographolide is about 85%.The thesis did related research on the extraction and purification of andrographis Paniculta lactone, and acquired better crafts methods on enhancing the extraction ratio,shortening extraction time, and improving the purification ratio, of andrographisPaniculta lactone compared with the traditional ones which makes it a beneficialreference for the future development and reduction of the side-effects of andrographisPaniculta lactone.