The Study Of Biodegradation On 4-Nonylphenolp

Nonylphenolp(NP)is a kind of compound which is hard to dispose under biology fathering,and has comparatively high toxicity to EEDs.The key point of washing waste water is the effective fathering of lignin.Through a large amount of experiment, such as the separation of high effect bacilli and its' growth trait,the degradation capability of high effect to 4-NP,and experiment of biology strengthen gene,the thesis provides theory support of improving the disposal effect of 4-NP in the washing waste water.1.Screening out 5 bacilli from 31 kinds of bacilli,which can grow well in the solid culture medium,making elementary research of the degradating capability of these 5 bacilli to 4-NP.2.Separating the animalcule which can grow comparatively well in the culture medium which includes 4-NP after domesticating the gathered sample.Screening out 5 bacilli from 31 kinds of bacilli after separating them though flat score,and marking them as strain A,strain B,strain C,strain D,strain E,all of them can grow well in the culture medium which includes 4-NP.We also make research of its shape character.3.Through the research of the growth characteristic of these bacilli,we find out that A,B,C,E come into stabilization 48 hours later,among them,A grows close to B,D enters into stabilization quicker than the other four,C proliferate cells faster than the other four in the same time.4.The environment has some effect on the growth of the bacilli.Under the condition of the lab,there exists difference among the 5 bacilli with different pH.A grows bad under pH5.0 and pH 9.0,while grows well under pH 6.0-8.0.B restrains its growth under pH5.0.A grows close to B under 20℃and 25℃.These two grow a litter bit high under 25℃.C grows well under the condition of alkalescent,the proper temperature is 35℃.However,D grows well in the two extremes, commonly under pH6.0-8.0.D restrains its growth under pH5.0,and the proper temperature for D and E is 20℃.5.Adopting the method of mutation law-ultraviolet irradiation mutagenic law,and making a series of disposal to the 5 bacilli by use of the appropriate mutagen dosage,we can get 3 bacilli X,Y,Z which grows well,making favorable foundation of degradation.The result indicates that the degradating capability of X and Y is higher than Z. 6.We do detailed research on the characteristic of the high effect bacilli,particularly under various environment condition.By studying the propagation of bacilli and the change of the capability to degrade 4-NP,we can find out the suitable condition.We can improve the efficiency of the high effect bacilli under these condition,wiping off 4-NP 76.4%.The result indicates that the condition of temperature is 30℃.7.Through the medium of manifold cometabolism primary carbon source and nitrogen source efficiency comare,we found that 4-NP degradation can be increased with the concentration of external carbon source for straint X and straint Y,but not the straint of Y.8.Under the optimal combination of temperature,pH.some laboratorial simulation experiments were carried out.The experimental results show that the performance of the group containing mutated strain is better the performance of CK.When the density of 4-NP was 100mg/L,the removal rate of mutated group was 75.2%after 1d degradation and that of CK was 5.1%.When the density of 4-NP was 300mg/L, the removal rate of mutated group was 67.5%after 1d degradation and that of CK was 3.9%.