Chronic Effects Of Cadmium Chloride On The Prostate And Seminal Vesicle Of Adult Rats

ObjectiveThe study was to explore the chronic effects of gastrointestinal exposure to cadmium chloride (CdCl₂) on histological structure and ultrastructure of prostate and seminal vesicle of rats. And to examine the changes of c-fos and c-jun expression in prostate and seminal vesicle with the effects of gastrointestinal exposure to Cd²⁺, in order to clarify the damage of Cd²⁺ on the prostate and seminal vesicle at the molecular level. Material and MethodsTwenty-four male Sprague-Dawley (SD) rats were randomly divided into six groups including the control groups (group A and B) and the experimental groups (group C, D, E, and F). The group C and D were given solutions containing Cd²⁺ 2.9mg/kg·day by gastric perfusion once a day, while group E and group F were given solutions containing Cd²⁺5.8mg/kg·day by gastrogavage once daily. The group C and E were administered for 2 months as subchronic exposure to Cd²⁺ respectively. The group D and F were administered for 4 months as chronic exposure to Cd²⁺. The prostate indexes and seminal vesicle indexes were detected and HE staining was used to observe changes on histological structure of prostate and seminal vesicle tissues. Transmission electron microscope was used to observe changes on ultrastructure of prostate and seminal vesicle tissues. The expression of c-fos mRNA and c-jun mRNA of prostate and seminal vesicle was detected by RT-PCR. Results Histological structure and ultrastructure of prostate: The prostate indexes...