| In recent years, environmental endocrine disrupting chemicals (EDCs) pollution has gradually become environmental problem. Natural estrogens such as 17β-estradiol (E2) and estrone (E1) are excreted daily by humans and animals, which are classified as endocrine-disrupting chemicals, are always present in sewage wastewater. They are more common in the natural world, which has a greater harm even in low concentrations, and have a huge impact on biotechnology. As China is a large agricultural country with the huge population, the environmental and ecological hazards problems which caused by the EDCs should not be neglected. To conduct an in-depth study is very necessary.For several years, the monitoring of natural estrogens at sewage treatment plants has been examined by many analytical methods. More analysis of the current methods usually requires large-scale equipments. The analysis needs high costs, pre-treatment technique and time-consuming operation. Therefore it is impossible to achieve a large amount samples analysis and real-time monitoring on it. Development of a simple and rapid detection method can greatly reduce the testing inputs and increase the capacity to monitor the environmental hormone. Surface plasmon resonance technique (SPR) is a new type immune sensing technology, compared with other methods, It's has some merits such as without labeling, real-time monitoring, etc.In this paper surface plasmon resonance based on biosensor was developed for monitoring natural marine environment estrogen. Using self-assembly Carboxymethyldextran chip, Small estrogen molecules was immobilized on the surface of sensor chip. Indirect competitive immunoassay method is adapted to detecte 17β-estradiol and estrone. The chip surface could be regenerated by certain conditions and then can be used reused; a sample measured only need 15 minutes. The limitation of detection for E2 and E1 is 0.445 and 0.668ng/ml respectively. Environmental samples... |
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