Analysis Of Steroid Estrogens And The Application Of Highefficiency Air-Lift Loop Bioreactor

Recently, the pollution of steroid estrogens has arisen the attention of many researchers overseas and they have begun to study the concentration and the biodegradation of these steroid estrogens in the environment. Based on the establishment of analytical method of steroid estrogen, the steroid estrogens in some contraceptive producing factory were measured.Though optimization, the optimized conditions of chromatogram and mass spectrum were determined. Though comparison of some common analytical method for steroid estrogens, it is found that HPLC/MS/MS is a rapid, selective and sensitive analytical method compared to other methods.Based on the established method, estrone(E1), 17β-estradiol(E2), estriol(E3) and EE2 in wastewater treatment plant(WTP) of a pharmacy factory, mainly producing contraceptive medicine in Beijing, China was determination. The average recoveries ranged from 88% to 103% and the precision of the method ranged from 9% to 4%. Based on 0.5 L wastewater samples, the limit of quantification (LOQ) was determined at 0.7 ng/L for E1, 0.8 ng/L for E2, 0.9 ng/L for E3, and 0.5 ng/L for EE2 in influent, and 1.0 ng/L for E2 and EE2, and 2.0 ng/L for E1 and E3 in effluent. In the influent samples of the bioreactor, average concentrations of 180±18, 85±9, 73±6.3 and 155±4.5 ng/Lwere determined for E1, E2, E3 and EE2, respectively, showing that they were removed in this biosystem. And in the effluent samples of the bioreactor, average concentrations of 56±5.3, 20±5.2, 10±4.4 and 40±6.0 ng/L were determined for E1, E2, E3 and EE2, respectively.In this study, laboratory-scale experiments were conducted out by using Triplet Loop Airlift Biofilm reactor (TLAB) to treat wastewater from pharmaceutical factory. In the steady phase, the removal rates of CODCr and ammonia were 73% and 70%, respectively. Biological nitrogen removal can be achieved via nitrification at 27-30oC, pH8.5. Meanwhile, the effect of HRT was investigated. The questions about the effect of pH and free ammonia on shortcut nitrification and nitrite-oxidizing bacteria adept to the free ammonia are also being discussed. The experimental results indicated that the removal rates of CODCr and ammonia were 65% and 50%, 73% and 70%, 75% and 72% when the HRT were 3h, 6h and 9h, respectively. The ratios of the nitrite accumulation were 90%, 80%, and 70%. In the whole period of the experiments, the effluent of the bioreactor kept steady. The experimental results can show that this bioreactor has high efficiency and high flexibility with respect to fluctuating loading rates.