Study On Security Of Daminozide And Gibberellic Acid Of Plant Growth Regulator

Daminozide and gibberellic acid which are plant growth regulators were reasearched in this article.The research mainly studied the influence of them upon a-amylase and a-glucosidase's activity in vitro digestibility by pancreatin, radicals and lipid peroxide, nitrosation reaction and inducement on micronucleus frequency in vicia faba root tips. The experiment results were as following: 1.Daminozide1.1 In vitro digestibility by pancreatin with pH6.8,37℃, 0.15% of the concentration of α-amylase and 2% of the concentration of dissolubility amylum, along with the reaction time, the acitivity of a-amylase was reduced by 26.93 % maximally by daminozide, when the concentration of daminozide was 200mg/L and the reaction time was 4h.In vitro digestibility by pancreatin with pH6.8, 37℃, 2.5mg/mL of the concentration of a-glucosidase and 0.116mol/L of the concentration of substrate, daminozide could increase a-glucosidase's activity by 47.42% maximally, when the concentration of daminozide was 40mg/L and the reaction time was 1h.1.2 Daminozide almost had no evident antioxidative effect on ·OH and O₂^- (P>0.05). But daminozide could accelerate LPO by 30.80% maximally , when the concentration of daminozide was 10mg/L.1.3 Daminozide could produce NO₃^- in vitro digestibility by pepsin with pH2.0, when the reation time was 30min. It could produce about 0.25mg/L NO_<sup>-3 , when the concentration of daminozide was 200mg/L.In vitro digestibility by pepsin with pH2.0 and 3mL 5ug/mL NaNO₂ in reaction system, the content of NO₂^- in reaciton system was reduced with the increase of the daminozide concentration and reaction time. When the concentration of daminozide was 200mg/L, the content of NO₂^- could be reduced by 57.81% 60 minute later.In vitro digestibility by pepsin with pH2.0, 0.5mL 1mmol/L NaNO₂ and 0.5mL 1mmol/L dimethylamine in reaction system, as the concentration of daminozide increased gradually, the capability of disconnecting of NDMA became reinforcing accordingly by 51.30%, when the content of daminozide was 200mg/L.