Study On The Separation And Purification Of Taxol

Separation and purification of taxol from Taxus Yunnanensis extract and fermentation liquor of yew endophyte were mainly carried out by adsorption chromatography.Because of large usage of organic solvent and emulsification , traditional liquor-liquor extraction was limited on some degree,especially on separation from fermentation liquor of yew endophyte. This thesis used resin chromatography insteadly as the first step,and HZ818 macroreticular resin was screened from six kind resins,it showed excellent effects of adsorption and separation. Meanwhile, single-factor and orthogonal experiments were carried out to optimize the technological parameters. Through this chromatography process,decoloration and purification were achieved,the even recovery of taxol was more than 98%.Normal-phase chromatography process was established as the second step. Silica gel and Al2O3 were chosen to study. Elutent composition,strength and rate of elution,rate of height and diameter were optimized. Results showed that the content of taxol was greatly increased after Al2O3 chromatography. It might be that some taxol-like taxanes converted to taxol under the catalyzing of Al2O3. Through resin and Al2O3 chromatography,the purity of taxol reached 30%, and the recovery reached about 125%.C18 reversed-phase chromatography was selected as the final step to further purify taxol. By optimization of eluent composition, strength of elution, elution mode and capacity of loading, with two column in series, the purity of taxol can be reached nearly 93-94%, followed with crystallization and recrystallization, the purity can increase to about 98%. Through this whole process, the total recovery of taxol was about 88%.The structure of taxol was identified with IR,HPLC and MS, which was identical with the results that reported on documents.