Disinfection Mechanism And Production Technology Of Acid Oxidation-Potential Water

Acid oxidation-potential water (AOW) was generated from electrochemical process, with the advantage of security, convenience, high-effectiveness, quickness and little residual toxicity. Despite of its effectiveness and potential of application, the disinfection mechanism of AOW is still an unsettled issue. There still have remained a key problem on the production of AOW, including low electrolysis efficiency and small yield scale, which may have hindered its further development and application. The purposes of this research were to explore the killing mechanism of AOW and optimize its generation process.In this study, the germicidal effect of AOW was demonstrated in the tests on Escherichia coli, colihage f₂ and spores of Bacillus subtilisvar Niger. The active oxygen concentration in the AOW and its contribution to disinfection were investigated. The synergistic effect of active oxygen and available chlorine was tested in anti-bacterial process. The comparison tests of different bactericidal methods were conducted and the morphology and structure of the cells was observed with the scanning electron microscope (SEM). Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin trapping technique was used for the detection of hydroxyl radicals. In addition, various factors affecting the production efficiency of AOW were studied. The following results were obtained:AOW had high efficiency for killing bacteria. For example, the killing ratio was more than 99.99% with contact time of 5min against E.coli, 30min and 60min respectively the latter two.Active oxygen played a key role in the disinfection process, and the synergistic effect was observed while co-existing with available chlorine.SEM observations indicated that after different means of disinfection, E.coli cells treated by AOW were similar to those treated by Fenton reagent, substantial leakage of intracellular materials was found.Hydroxyl radical OH was detected from the AOW, and it might come from the interaction of active substance. Hence it can be hypothesized from the experiments performed that perhaps the significant killing action of AOW was due to the formation of short-lived and high-energy intermediates and/or end-products such as hydroxyl radical.Via the optimization of the reactor design, high-iridium-contented DSA...