Study On Fe～(3+) Reduction Properties And Azo Dye (the Cause Of Non-infectious Disease) Decolorization Of Decolorationis S12～T

A new species of Shewanellade decolorations S12 was isolated from activated sludge of a textile-printing waste-water treatment plant . In the anaerobic condition, S12 could conserve energy for growth by using Fe³⁺ as the terminal electron acceptor. At the optimal condition of pH8, temperature 30℃, ferric citrate 800mg/L, sodium lactate 2g/ L and yeast extract 0.5g/ L , the cell growth increased with the raise of the amount of the Fe³⁺ reduction in 8h. The effect of different carbon soucres, nitrogen sources,pH values and growth temperatures on the anaerobic Fe³⁺ reduction of Shewanella decolorationis S12 was investigated. LB was favorable for Fe³⁺ reduction. Glucose and sodium lactate also were favorable for Fe³⁺ reduction. The cell growth and Fe³⁺ reduction increased with the raise of the amount of the yeast extract from 0 to 4g/L.The amounts of the sodium lactate of 6g/ L and ferric citrate of 800mg/L were suitable for strain S12 growth and Fe³⁺ reduction. In the investigated initial pH value range of 6⁸,Strain S12 growth increased with the raise of the pH value. The optimum pH value range for Fe³⁺ reduction was pH6⁸. strain S12 could growth and reduce Fe³⁺ at the temperature range of 20⁴0℃. The best temperature for strain S12 growth and Fe³⁺ reduction was 30℃.It was discoveried from the study of the connection of Fe reduction and azo dye decolorization of Shewanella, it could advance azo dye decolorization when adding Fe³⁺.In the amount of the ferric citrate range of 0¹400mg/L, the strain S12 azo dye decolorization increased with the raise of the amount of the ferric citrate. At the same time, in the amount of the azo dye range of 0²00mg/L, the azo dye decolorization increased with the raise of the amount of the azo dye when adding ferric citrate.. Azoreductases and Fe³⁺ reductases were preliminary established on the cell membrane.Waster water containing azo dye of 100 mg/L was used to irrigate rice plants in pot experiments and its poison effects on the rice plants was evalutated. The results showed that plant height, ear length, ear weight, thousand kernels weight, kernels number per ear and maturing rate rice were lower in the treatment by irrgation than those by routine irrigation. SAS software analysis indicated that there was significant difference between the azo dye waste water irrigation and the convention water irrigation. The yield decreasing...