| In this paper, we evaluated the individual and the combined antioxidant capacities of the typical composed-antioxidants-TBHQ BHT BHA PG. In addition, a new method to evaluate total antioxidant power of samples was posed firstly in domestic and the factor of this reaction system was investigated.In series I , the individual antioxidant activity of TBHQ BHT BHA PG was studied by DPPH assay. The results illustrated that: all of the four composed-antioxidant had significant activity of scavenging DPPH radical ;compared with the control group, different concentration of the every antioxidant had significant activity of scavenging DPPH radical in a dose-dependent manner(P < 0.05); The IC50 of TBHQ, BHT, BHA, PG were 24.789μmol/L, 46.478μmol/L,35.772μmnol/L, 28.699μmol/L separately; the results showed that the scavenging ability of DPPH radical from the highest to the lowest was TBHQ, PG, BHA, BHT.In series II, the antioxidant activities of the mixed antioxidants-TBHQ+BHT, TBHQ+BHA, BHT+PG, BHT+BHA, PG+BHA were studied by DPPH assay. The results illustrated that: for the five kind of mixed-antioxidant, the scavenging ability of DPPH radical of had raised with the concentration of any of their components ; every component had an positive effect on the antioxidant activity of the mixed-antioxidants , the higher-concentration component had more dominant effect on the antioxidant capacity of the mixed-antioxidant, the two component of the mixed-antioxidant had synergistic effects .In series III, the FRAP assay was used to measure the total antioxidant power of TBHQ, BHT, BHA, PG . The results showed that: The FRAP values of every antioxidant was related to the concentration of them; in the same FRAP values (0.5mmol), the concentration of TBHQ, BHT, BHA, PG were 0.6119mmol/L, 6.6756 mmol/L, 0.6239 mmol/L, 0.5859 mmol/L separately ;the order of the antioxidant capacity from the highest to the lower was PG,TBHQ,BHA,BHT. |
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