Study On Using Endophytic Fungi Of Sinopodophyllum Emodi (Wall.) Ying. To Produce Podophyllotxin

Podophyllotoxin is a kind of anticancer and insecticidal lignans. It is an effective medicine in treating wart and a precursor of some anti-tumor medicine. Because of its great medical potential, podophyllotoxin has been demanded desperately. However, the natural resource of it is very limited. Therefore, how to utilize and develop its resource reasonably is a hot point.The experiment tries to obtain podophyllotoxin through microbial fermentation to settle the problem of resource shortage. An endophytic fungi Iml was isolated from Sinopodophyllum emodi (Wall.) Ying . The fermentation production was extracted by chloroform and /rbutanol and the extraction was collected respectively. Three compounds were separated from two kinds of extract through silica gel C. C. (200-300 mesh) , TLC and recrystallization. Compound I was identified as podophyllotoxin and compound II as kaempferol, compound III was initially considered as a kind of podophyllotoxin- 0- Glu type on the basis of TLC, HPLC and ¹H, ¹³C-NMR. The experiment also studied the biological character of the strain Iml. The initial result shows that Iml belongs to Fungi Imperfecti, Moniliales, Dematiaceae, Alternaria Nees ex Wall. Iml grows well in PDA medium and has a good genetic stability. The optional fermentation condition of Iml was also investigated: PH 6.0—6.5, temperature 25°C.The result fully indicates that it is feasible to use endophytic microbial of anti-cancer medicinal plant to produce anticancer substance. The research pioneer a new gateway for the development of medicine resource, and it has a very important meaning to protect biological environment.