The Study On DNA Damage Of Freshwater Crab (Sinopotamon Yangtsekiense) Resulting From Cadmium And Lead

Cadmium(Cd~2+), lead(Pb~2+) and hydrargyrum without physiological function have an influence on growth of biology and possess distinct toxicity, which is named heavy metal pollution. This kind of pollution exhibits the following characteristics: wide source, long time with toxicity, easily cumulating, transfer along with food chain and hardly resuming etc. Aquatic environmental pollution resulting from heavy metal is a serious and growing problems, with the increasing number of industrial, agricultural and commercial chemicals in the aquatic environment having led to various deleterious effects on organisms. The impact of heavy metal on the integrity and functioning of cellular DNA has been highly regarded. Heavy metal pollution leads to DNA damage, which can place a premium on cellular mutation and cancer. Accordingly, the cellular mutation and cancer can be transmitted to the next generation cells to result in functional loss and even aberrance.The paper reported the DNA damage effect of Cd2+ and Pb2+ on the freshwater crab Sinopotamon yangtsekiense under different concentration conditions with the single cell microgel electrophoresis (SCGE) technique from August in 2003 to May in 2005. The crab were treated with lower concentrations (Cd2+: 0.1, 0.2, 0.3, 0.4 and 0.5 mg/L; Pb2+: 0.05, 0.10, 0.15, 0.20 and 0.25 mg/L) and higher concentrations (Cd2+: 1.21, 3.83, 12.13, 38.34 and 121.17 mg/L) for 7 days. Simultaneously, we adopted chronic disposal containing 0.01 mg/L for 40 days and 0.005 mg/L for 20 days. In chronic disposal process, spermary cells were detected with SCGE technique at 5 days intervals. Mathematics analysis curve was protracted on the basis of experimental data. The results indicated that spermary cells in lower and higher concentrations groups exhibited DNA damage. The effect of DNAdamage changed with concentration of Cd , Pb and was positive relationship to concentration of Cd , Pb . The female crab was more highly influenced compared to male crab by this kind of DNA damage effect. The...