| There are abundant locust resources in our country and an elementary estimation is that there are more than 1000 kinds of locusts. At present, utilization about locust is focused on the functional analysis of proteins, such as some antibiotic peptide, amino acid, etc. but the study on the functional analysis of the grease of Oxyachinensis Thunb.is scarce. This article does some systematic research on the grease of Oxya chinensis (Thunberg), and also does some functional evaluation on the grease. In addition, this article extracts and isolates phospholipid of the grease. And thus it provide theoretic base for the exploration and utilization of the grease of Oxya chinensis (Thunberg).The composition of fatty acids in the grease of Oxya chinensis (Thunberg) was analysed by GC. The result was that it consisted mainly of palmitic acid 7.8%, palmitoleic acid 3.0%, stearic acid 3.7%, oleic acid 29.1%, linoleic acid 25.0%, linolenic acid 30.6%. The unsaturated fatty acid amounted to 87.7%. Physical and chemical testing results showed that acid value is 4.5, saponitication value is 171.6, iodine value is 138.9 and POV is 0.0412%(it is 0.2011% stored in a month).After a severe examination of several sorts of extraction solvents, the grease contents of Oxya chinensis (Thunberg) was tested in the traditional SUOSHI extraction way, and the results showed that light petroleum (the range of boiling point is 30-60) was optimal for the extraction, and the grease contents of Oxya chinensis (Thunberg) was 7.47%. At the same time, this article also studied several other methods on the extraction of the grease of Oxya chinensis (Thunberg), such as the way of quench, the extraction way of acidic aether, the extraction of the n-hexane, the way of CM and the method of ultrasonic wave treatment. The results showed that ultrasonic treatment was a rather fitting approach to extract the grease of Oxya chinensis (Thunberg). The powder of Oxya chinensis (Thunberg) was treated byultrasonic for enchancing the extraction rate of its grease. The effect of several factors (ultrasonic wave intensity, treatment time, treatment times) on ultrasonic processing was explored, and the optimal conditions of ultrasonic processing were obtained. As a result, it was under such conditions (ultrasonic intensity 500W, treatment time 3 sec, one time treatment times 120) that extraction rate of the grease of Oxya chinensis (Thunberg) was better.In the separate way of organic solvent and in the way to hydrate and unglue the phospholipid in the grease of Oxya chinensis (Thunberg) was isolated. The results showed the separate way of organic solvent was better. The effect of several factors (extraction time, the temperature of dipping in the acetone, time of dipping in the acetone, the ratio of the sample and the acetone) on the separate way of organic solvent was explored. The optimal conditions of the separate way of organic solvent were obtained. The results showed that it was under such conditions (extraction time 6h, the temperature of dipping in the acetone 30℃, time of dipping in the acetone 3h, the ratio of the sample and the acetone 500mL/lg) that separation rate of phospholipid in the grease of Oxya chinensis (Thunberg) was better.This paper firstly researched extraction, isolation and composition of locust phospholipids. It showed that the content of phospholipids was 7.85%, and there were PC 75.0%, PE 11.3%, sphing omyelin 8.13% by organic solvent extraction and column chromatogram.The function evaluation on the grease of Oxya chinensis (Thunberg) was made through pouring stomach for the high fat rat group. The subjects were 50 ICR Male rat and were divided into three dose group, a contrast group and a high fat rat model group. These subjects were used to study the function of reducing blood fat of the grease of Oxya chinensis (Thunberg). The results showed that the level of the serum TG was far lower than that of high fat rat model group (middle dose group and high dose group t>t_α,0.05) and that the level of the serum TC was even lower than that of high fat rat model group(low dose group, middle dose group and high dose group... |
