| Nano science and technology (Nano S&T) is an interdisciplinary area studying substance with single atom or molecule. Nano S&T is based on varieties of modern advanced science and technology (chaos physics, quantum mechanics, mesoscopic physics, molecular biology, computer technology, microelectronics, scanning tunneling microscopic technology and nuclear analytical technology). Nano S&T is initiating a series of new science and technology, such as nano-electronics, nano-materials, nano-mechanics, et al. In one word, Nano S&T is one of the most advanced science and technology for the new century.Nano materials are made up of nanoscale materials, such as particles. Nano-particles' 3-D dimension is in the scale of 1100 nanometer, which is in the transition region of the dimension of boundary of atom cluster and macroscopic object. From the general view of microcosmic and macroscopic aspects, such system is a typical mesoscopic system, not a typical microcosmic system or a typical macroscopic system. They have very interesting characters, such as surface effect, litter size effect, quantum measurement effect and macroscopical quantum tunnel effect. When the materials is in nanometer magnitude, it will have remarkable difference compared with its bulk materials on its optical, thermal, electrical, magnetic, mechanic and chemical properties. The high specific surface area, high catalytic activity, special physical and chemical properties, super minuteness of the nanomaterials made them very promising materials of chemically modified electrodes.Recently, life science has attract researchers wide attention——smallmolecular active substance in live bodies. For examples, some thiol compounds have an important effect on the procedure of the live bodies. Protein also works as a large message molecule, having the key function in the live bodies. So the relation between the determination of them and their electrochemical behavior has an important significance. In the thesis, we prepared some new kinds of nanomaterials,which are characterized by modern analytical methods. Selected nanomaterials were used to modify electrode and the modified electrode was used as electrochemical detector. Combined with chromatograph and microdialysis, the detector was successfully used to detect some active substance in rat brain in vivo. Some other nanomaterials were selected to modify electrode and the electrochemical behavior of proteins were studied. Combined with flow injection analysis, the concentration of real blood samples was detected. The work of the thesis is given as followers:1. Preparation of a nanoparticle cobalt hexacyanoferrate film chemically modified electrode and its application to determine L-cysteine and glutathione in rat striatal microdialysatesA method to prepare a nanoparticle Cobalt Hexacyanoferrate (CoHCF) film chemically modified electrode (CME) was developed. And the electrocatalytic oxidation of L-cysteine and glutathione on the nanosized CoHCF CME was studied. Coupled with high performance liquid chromatograhpy, the electrode is utilized for electrochemical detector for L-cysteine and glutathione; The peak currents of L-cysteine and glutathione were linear to their concertrations ranged from 2.0*10-7 to 2.0*10-4mol/L, and the correlation coefficients were 0.9998 with the detection limits of 9.0*10-8mol/L and 1.2*l0-7mol/L,respectively. The method had been successfully applied to assess the contents of L-cysteine and glutathione in rat striatal microdialysates.2. Preparation of a nanosized CoHCF modified electrode and its application to determine hemoglobinA Cobalt Hexacyanoferrate (CoHCF) nanoparticle chemically modified electrode (CME) was fabricated and the direct electrochemical behavior of hemoglobin (Hb) at this nanosized CoHCF CME was studied. A new method to detect Hb with nano-material chemically modified electrode was established in the thesis. The results showed that the electrode performed efficiently electrocatalytical reduction for Hb. Combined with the pump of liquid chromatograp... |
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