Nitrile Converzymes And Surface Modification On Polyacrylic Fiber

Enzyme is specific and efficient for catalysis and it is environmentally friendly, so its applications in textile and dyeing process have a broad future. The enzymatic way for modification of polyacrylic fibers was compared with those traditional ones and their characters were described. In this study, an application of nitrile converzymes on the surface modification of polyacrylic fibers was investigated.The primary principle of enzymatic modification of polyacrylic fibers is that the nitrile groups on the surface of acrylic fibers could be selectively converted to the corresponding amidic groups by nitrile converzymes. The work in this paper could be mainly divided into four parts.Firstly, we investigated the factors influencing the enzymatic catalysis. The results showed that propionitrile was better than acetonitrile as a substrate, and the optimal substrate concentration should be 0.02 g/1. In order to measure the activity of nitrile converzymes more precisely, a gas chromatographic method was used to compare with a spectrocolorimetric method. A product of the enzymatic reaction of nitrile,acetamide was detected by gas chromatography, so that the existence of nitrile hydratase in nitrile converzymes had been confirmed.Secondly, the culture condition of Arthrobacter sp. Jlwas optimized to promote the activity and production of nitrile converzymes. The following condition, 3.0g/l glucose, 4.0g/l extract and 4.0 g/1 peptone was found to be the most suitable carbon and nitrogen sources. Since nitrile converzymes from Arthrobacter sp. Jl were inducible, some researches on its inducible property were made. 0.4% acetonitrile was added to the culture medium at the begin of cultivation, and it did not inhibit the growth of Arthrobacter sp. Jl. The optimal temperature for its growth was 35 ℃, however, for synthesis of nitrile converzymes, The optimal temperature was 30℃.Thirdly, to extract the enzyme, the conditions for disruption of Arthrobacter sp. Jl cells by ultrasonic was studied and optimized. The pulse time of ultrasonic should be 10 minute, and the power was about 360 watt. The concentration of cells was 20 %.Finally, enzymatic modification used the nitrile converzymes and pretreatment of plasma for polyacrylic fibers were studied. Dyeing was used to evaluate the result of enzymatic modification of polyacrylic fibers. The result indicated that the pretreatment of plasma could enhance the accessibility of polyacrylic fibers to enzymes and then improve the effect of enzymatic modification.