On Application Of Resonance Light Scattering In Water Environmental Monitoring

The article concluded resent research actuality and history in Resonance light scattering (RLS) field, respectively introduced the important achievement in theoretical research and the work in applicable research in RLS field.The article also deeply studied the determinative methods of Plumbum, Cadmium and Anionic Surfactants(ASF) in environmental water samples by RLS and builded new correlative analytical methods. The article also used RLS to study other work in the correlative fields. Furthermore,the article also studied the combinative number of protein and biological dyestuff Congo red in different pH B-R suffer solution and gave the combinative model of biological big molecular and dyestuff ,that was Scatchard model, and used mole ratio method to acquire the combinative number of protein and Congo red.The article used RLS to determinate Plumbum in environmental water samples, on basis of enhanced effect with Plumbum (II)-BSA-Congo red system, new method of determining Plumbum (II) was founded. The optimum reaction conditions and the quantitative relationships of the intensities of RLS to the concentration of Plumbum were established. The RLS intensity at 575nm was respective maximum and proportional to the concentration of Plumbum .The method was simple and rapid with a detection limit of 0.358pμg/ml and a linear range of 0.0~7.0μg/mL.It has been applied to the determination of Plumbum in water with satisfactory results in contrast with atomicabsorption method. Based on Pb2+ and G2O72" to form hydrophobic sol system which enhances the RLS intensity of this system, Resonance light scattering turbidimetry (RLST) determinated Plumbum in environmental water samples. The optimum conditions and the quantitative relationships were established. The RLS intensity at 497nm was respective maximum and proportional to the concentration of Plumbum.The detection limit was 0.057ag/ml and linear range was 0.1~l.GjMg/mL. It has been applied to the determination of Plumbum in water with satisfactory results in contrast with atomic absorption method.The article used the method to determinate Cadmium in environmental water samples based on Cd2+ and S2"to form hydrophobic sol system. The optimum reaction conditions and the quantitative relationships were studied. A Resonance light scattering peak at 476nm was found and was proportional to the concentration of Cd2+ .The linear range was 0.0~20.0,Mg/ml with the detection limit of 6.33xlO'Vg/ml for the Cd2+.The RSD and Recovery was 1.2%~1A%, 99.80%~102.30% respectively. The method was simple and rapid and sensitive. It has been applied to the determination of Cd2+ of in environmental water samples and the result was satisfactory in contrast with atomic absorption method.The article used the method to determinate Anionic Surfactants(ASF) in environmental water samples, A new method has been developed based on ASF reacting with Rhodamine B to form the ion association complexes in pH 3.0 (SDS system) or pH 3.0 (SDBS system) buffer solution, which resulted in increase of resonance light scattering intensity. The maximum RLS peaks was at 518nm for RhB-SDS complex and 373nm for RhB-SDBS complex, respectively.The method had high sensitivity and the detection limits were 0.023Jag/mL for SDS and 0.038,Mg/mL for SDBS.This method was applied to the determination of anionic surfactants in synthesized water samples and environmental water samples with a recovery from 94.0% to 105.0% and compared with Methylene blue method with a satisfactory result. This method was simple and quick to determinate anionic surfactants in environmental water.Besides, the article also used RLS to study protein-Congo red system and Protein-Xylenol orange system and determinate human serum protein with satisfactory results.