Immunoassay Method For The Analysis Of Insecticide Diflubenzuron

Diflubenzuron have desirable features and potential for use as insecticides, it has high insecticidal characteristic and low toxicity.And it has important value about how to analyse the residues in environment. In order to obtain this information,the capacity to analyse a large number of samples should be available. But the classical methods of diflubenzuron analysis by GC or HPLC are expensive and tedious, although they are of adequate sensitivity. Improved analytical methods are needed so that a large number of samples can be inexpensively analysed within a short period of time. Because immunoassay for pesticide analysis is simple, cost effective, and adaptable to laboratory or field, it has been proved to be the best choice for the analysis of the large number of samples.Since it was first used in 1971,immunochemical methods have been rapidly gained acceptance as analytical techniques for pesticide analysis. This paper described the development of sensitive competitive enzyme-linked immunosorbent assays (ELISAs) for quantitative detection of insecticide diflubenzuron in soil and water. Two haptens were synthesised for the production of diflubenzuron antibodies and used in the enzyme conjugate. After analysed, the best antibody was chosen and the developed assay was very specific to diflubenzuron. In the paper, a highly sensitive and specific ELISA method is described.No significant matrix effects from soil (90% methanol extraction), water and other pesticide residues were observed for the developed assay. The detection limit of this assay was 0.05 μ g/dm~3, and more than 80% recovery was obtained for diflubenzuron in soil and water recovery studies.