Extraction And The Antioxidant Stability Of Ascidian

The oil was extracted from Ascidian with supercritical fluid CO2 and ethyl acetate , and the antioxidant activity of the oil was studies with OSI. The oil extracted with supercritical fluid CO2 is more pure than that with ethyl acetate, and it contains more EPA and DHA, its antioxidant stability is also higher, its Ip is 13.9h(Stye/a clava) and 15.9h(Ciona intestinalis Linnaeus) at 40℃. Some antioxidants were added into these oil to improve their antioxidant stability. The extraction from Tanshen and salvia plebeia with ethyl acetate have strong antioxidant activity: when added 0.04%, the antioxidant stability of oil increased 8-10 times, and the antioxidant activity is stronger in the oil extracted with ethyl acetate than with supercritical fluid CO2.1. The effect of several material disposal methods and extracting technics were studied on the quality of lipid extracted from Styela clava. The results showed that lipid's quality were influenced greatly by both material disposal methods and extracting technics . Ventilation airing was the best measure to deal with raw material, while extracted with super critical CO2 could gain lipid with high quality than organic solvent ethyl acetate.2. The extraction from Tanshen with ethyl acetate have strong antioxidant activity: when added 0.04%, the antioxidant stability of oil increased 8~10 times, and the antioxidant activity is stronger in the oil extracted with ethyl acetate than with supercritical fluid CO2.The antioxidant activities of natural components extracted from Salvia miltiorrhiza are investigated on the oil extracted from ascidian. Seven components are identified by chemical and spectroscopic methods as tanshinone I (1), tanshinone II A (2), cryptotanshinone (3), dihydrotanshinone (4), tanshinone II B (5), przewaquinone (6) and oleanolicacid (7). Their antioxidant activities are investigated and compared with VE by an oxidative stability instrument(OSI). The results show that components 4 and 1 have strong antioxidant activity at 0.01% levels.3. The antioxidant activities of natural components extracted from Dalbergia odorifera T. Chen on lard and ascidian oil were investigated by OSI. Dalbergia odorifera T. Chen was extracted with 95% ethanol, and the extract was then extracted by petroleum ether, CHCI3, EtOAc and BuOH successively. The residue of EtOAc had very good antioxidant activity. Eight components were isolated from it , i.e. , 2 , 4-dthydroxy-5-methoxybenzophenone(1); 2, 3, 7-trihydroxy-4-methoxy-isoflavanone(2); 3-methoxydai- dzin(3); 4', 5,7-trihydroxy-3- methoxyflavone(4); vestitol(5); medicarpin(6); 2-propenyl hexanoate (7) and ethyl hexadecanoate (8). The results showed that components 1,3,5 and 6 had antioxidant activity and components 2 and 4 had strong antioxidant activities at 0.02% and 0.04% levels both on lard and ascidian oil.