The Extraction Of Active Components Of Rubdosia Rubescens Hara And Relatives Studies

In this thesis, the chemical constituents of Rubdosia rubescens (Ldbiatase) Hara have been investigated further and the derivatives of ent-kaurene diterpene, which have a-methylene cyclopentanone function group in their molecule and show the antitumor activity, have been designed and prepared. In addition, for the better application of Acetylkamebakaurinin, we studied the preparation method of the inclusion complex of Acetylkamebakaurinin- 3 -cyclodexitrin. The detailed contents are shown as followings:1 By investigating different processes for the extraction of Rabdosia rubescens Hemsl, we found that the main constituents of Rabdosia rubescens Hemsl harvested in different areas or in same area but differ period manifested great difference. What's more, by doing that, we confirmed the best extraction time and we studied the affection of alcohol's concentration on the extraction efficiency of Rabdosia rubescens Hemsl.2, We studied the chemical constituents of Rabdosia rubescens Hemsl and isolated eleven compounds from the alcohol extraction of the dry leaves of Rabdosia rubescens Hemsl. Of which there are four compounds Acetylkamebakaurinin , Kamebakaurinin. sigmasterol and 3',4',5-trihydroxy-6,7-dimemoxyflavone which have been isolated from this plant for the first time. For compound (1), we first get it's crystal structure. The other seven compounds are known before. They are 3 -sitosterol, oridonin, ponicidin, pedalidin, lasiodonin, ursolic acid and 2 ,3 -dihydroxy-urs-12-ene-28-oic acid respectively.3m 7, 14-acetal derivatives of oridonin and Acetylkamebakaurinin were designed and synthesized and oridonin -6-O- -D-glucopyranoside were prepared via four steps. All the structures were characterized by elemental analysis, IR, and NMR spectra.4, We obtained the inclusion complex of Acetylkamebakaurinin- -cyclodexitrin via abrasive method, which improved the solubility in water.5, In order to make our project go well, we isolated and purified enough amounts of oridonin and ponicidin .In a word, in this thesis, the process for the extraction of Rabdosia rubescens Hemsl, the isolation , the synthesis of it's derivatives and the preparation of the inclusion complex of Acetylkamebakaurinin- -cyclodexitrin were studied. And partial of the chemical constituents and derivatives were tested for antitumer activities.